Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro

Yuji Haraguchi, Tatsuya Shimizu, Tadashi Sasagawa, Hidekazu Sekine, Katsuhisa Sakaguchi, Tetsutaro Kikuchi, Waki Sekine, Sachiko Sekiya, Masayuki Yamato, Mitsuo Umezu, Teruo Okano

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

The fabrication of 3D tissues retaining the original functions of tissues/organs in vitro is crucial for optimal tissue engineering and regenerative medicine. The fabrication of 3D tissues also contributes to the establishment of in vitro tissue/organ models for drug screening. Our laboratory has developed a fabrication system for functional 3D tissues by stacking cell sheets of confluent cultured cells detached from a temperature-responsive culture dish. Here we describe the protocols for the fabrication of 3D tissues by cell sheet engineering. Three-dimensional cardiac tissues fabricated by stacking cardiac cell sheets pulsate spontaneously, synchronously and macroscopically. Via this protocol, it is also possible to fabricate other tissues, such as 3D tissue including capillary-like prevascular networks, from endothelial cells sandwiched between layered cell sheets. Cell sheet stacking technology promises to provide in vitro tissue/organ models and more effective therapies for curing tissue/organ failures.

Original languageEnglish
Pages (from-to)850-858
Number of pages9
JournalNature Protocols
Volume7
Issue number5
DOIs
Publication statusPublished - 2012 May

Fingerprint

Tissue
Fabrication
In Vitro Techniques
Cell Engineering
Preclinical Drug Evaluations
Regenerative Medicine
Tissue Engineering
Cell- and Tissue-Based Therapy
Endothelial cells
Tissue engineering
Cell culture
Cultured Cells
Curing
Endothelial Cells
Screening
Technology
Cells
Temperature
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Haraguchi, Y., Shimizu, T., Sasagawa, T., Sekine, H., Sakaguchi, K., Kikuchi, T., ... Okano, T. (2012). Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro. Nature Protocols, 7(5), 850-858. https://doi.org/10.1038/nprot.2012.027

Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro. / Haraguchi, Yuji; Shimizu, Tatsuya; Sasagawa, Tadashi; Sekine, Hidekazu; Sakaguchi, Katsuhisa; Kikuchi, Tetsutaro; Sekine, Waki; Sekiya, Sachiko; Yamato, Masayuki; Umezu, Mitsuo; Okano, Teruo.

In: Nature Protocols, Vol. 7, No. 5, 05.2012, p. 850-858.

Research output: Contribution to journalArticle

Haraguchi, Y, Shimizu, T, Sasagawa, T, Sekine, H, Sakaguchi, K, Kikuchi, T, Sekine, W, Sekiya, S, Yamato, M, Umezu, M & Okano, T 2012, 'Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro', Nature Protocols, vol. 7, no. 5, pp. 850-858. https://doi.org/10.1038/nprot.2012.027
Haraguchi, Yuji ; Shimizu, Tatsuya ; Sasagawa, Tadashi ; Sekine, Hidekazu ; Sakaguchi, Katsuhisa ; Kikuchi, Tetsutaro ; Sekine, Waki ; Sekiya, Sachiko ; Yamato, Masayuki ; Umezu, Mitsuo ; Okano, Teruo. / Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro. In: Nature Protocols. 2012 ; Vol. 7, No. 5. pp. 850-858.
@article{85247160a83a4fa8b3bd4875ab233d04,
title = "Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro",
abstract = "The fabrication of 3D tissues retaining the original functions of tissues/organs in vitro is crucial for optimal tissue engineering and regenerative medicine. The fabrication of 3D tissues also contributes to the establishment of in vitro tissue/organ models for drug screening. Our laboratory has developed a fabrication system for functional 3D tissues by stacking cell sheets of confluent cultured cells detached from a temperature-responsive culture dish. Here we describe the protocols for the fabrication of 3D tissues by cell sheet engineering. Three-dimensional cardiac tissues fabricated by stacking cardiac cell sheets pulsate spontaneously, synchronously and macroscopically. Via this protocol, it is also possible to fabricate other tissues, such as 3D tissue including capillary-like prevascular networks, from endothelial cells sandwiched between layered cell sheets. Cell sheet stacking technology promises to provide in vitro tissue/organ models and more effective therapies for curing tissue/organ failures.",
author = "Yuji Haraguchi and Tatsuya Shimizu and Tadashi Sasagawa and Hidekazu Sekine and Katsuhisa Sakaguchi and Tetsutaro Kikuchi and Waki Sekine and Sachiko Sekiya and Masayuki Yamato and Mitsuo Umezu and Teruo Okano",
year = "2012",
month = "5",
doi = "10.1038/nprot.2012.027",
language = "English",
volume = "7",
pages = "850--858",
journal = "Nature Protocols",
issn = "1754-2189",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro

AU - Haraguchi, Yuji

AU - Shimizu, Tatsuya

AU - Sasagawa, Tadashi

AU - Sekine, Hidekazu

AU - Sakaguchi, Katsuhisa

AU - Kikuchi, Tetsutaro

AU - Sekine, Waki

AU - Sekiya, Sachiko

AU - Yamato, Masayuki

AU - Umezu, Mitsuo

AU - Okano, Teruo

PY - 2012/5

Y1 - 2012/5

N2 - The fabrication of 3D tissues retaining the original functions of tissues/organs in vitro is crucial for optimal tissue engineering and regenerative medicine. The fabrication of 3D tissues also contributes to the establishment of in vitro tissue/organ models for drug screening. Our laboratory has developed a fabrication system for functional 3D tissues by stacking cell sheets of confluent cultured cells detached from a temperature-responsive culture dish. Here we describe the protocols for the fabrication of 3D tissues by cell sheet engineering. Three-dimensional cardiac tissues fabricated by stacking cardiac cell sheets pulsate spontaneously, synchronously and macroscopically. Via this protocol, it is also possible to fabricate other tissues, such as 3D tissue including capillary-like prevascular networks, from endothelial cells sandwiched between layered cell sheets. Cell sheet stacking technology promises to provide in vitro tissue/organ models and more effective therapies for curing tissue/organ failures.

AB - The fabrication of 3D tissues retaining the original functions of tissues/organs in vitro is crucial for optimal tissue engineering and regenerative medicine. The fabrication of 3D tissues also contributes to the establishment of in vitro tissue/organ models for drug screening. Our laboratory has developed a fabrication system for functional 3D tissues by stacking cell sheets of confluent cultured cells detached from a temperature-responsive culture dish. Here we describe the protocols for the fabrication of 3D tissues by cell sheet engineering. Three-dimensional cardiac tissues fabricated by stacking cardiac cell sheets pulsate spontaneously, synchronously and macroscopically. Via this protocol, it is also possible to fabricate other tissues, such as 3D tissue including capillary-like prevascular networks, from endothelial cells sandwiched between layered cell sheets. Cell sheet stacking technology promises to provide in vitro tissue/organ models and more effective therapies for curing tissue/organ failures.

UR - http://www.scopus.com/inward/record.url?scp=84859579818&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859579818&partnerID=8YFLogxK

U2 - 10.1038/nprot.2012.027

DO - 10.1038/nprot.2012.027

M3 - Article

VL - 7

SP - 850

EP - 858

JO - Nature Protocols

JF - Nature Protocols

SN - 1754-2189

IS - 5

ER -