Abstract
The fabrication of 3D tissues retaining the original functions of tissues/organs in vitro is crucial for optimal tissue engineering and regenerative medicine. The fabrication of 3D tissues also contributes to the establishment of in vitro tissue/organ models for drug screening. Our laboratory has developed a fabrication system for functional 3D tissues by stacking cell sheets of confluent cultured cells detached from a temperature-responsive culture dish. Here we describe the protocols for the fabrication of 3D tissues by cell sheet engineering. Three-dimensional cardiac tissues fabricated by stacking cardiac cell sheets pulsate spontaneously, synchronously and macroscopically. Via this protocol, it is also possible to fabricate other tissues, such as 3D tissue including capillary-like prevascular networks, from endothelial cells sandwiched between layered cell sheets. Cell sheet stacking technology promises to provide in vitro tissue/organ models and more effective therapies for curing tissue/organ failures.
| Original language | English |
|---|---|
| Pages (from-to) | 850-858 |
| Number of pages | 9 |
| Journal | Nature Protocols |
| Volume | 7 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2012 May |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
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Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro. / Haraguchi, Yuji; Shimizu, Tatsuya; Sasagawa, Tadashi; Sekine, Hidekazu; Sakaguchi, Katsuhisa; Kikuchi, Tetsutaro; Sekine, Waki; Sekiya, Sachiko; Yamato, Masayuki; Umezu, Mitsuo; Okano, Teruo.
In: Nature Protocols, Vol. 7, No. 5, 05.2012, p. 850-858.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro
AU - Haraguchi, Yuji
AU - Shimizu, Tatsuya
AU - Sasagawa, Tadashi
AU - Sekine, Hidekazu
AU - Sakaguchi, Katsuhisa
AU - Kikuchi, Tetsutaro
AU - Sekine, Waki
AU - Sekiya, Sachiko
AU - Yamato, Masayuki
AU - Umezu, Mitsuo
AU - Okano, Teruo
PY - 2012/5
Y1 - 2012/5
N2 - The fabrication of 3D tissues retaining the original functions of tissues/organs in vitro is crucial for optimal tissue engineering and regenerative medicine. The fabrication of 3D tissues also contributes to the establishment of in vitro tissue/organ models for drug screening. Our laboratory has developed a fabrication system for functional 3D tissues by stacking cell sheets of confluent cultured cells detached from a temperature-responsive culture dish. Here we describe the protocols for the fabrication of 3D tissues by cell sheet engineering. Three-dimensional cardiac tissues fabricated by stacking cardiac cell sheets pulsate spontaneously, synchronously and macroscopically. Via this protocol, it is also possible to fabricate other tissues, such as 3D tissue including capillary-like prevascular networks, from endothelial cells sandwiched between layered cell sheets. Cell sheet stacking technology promises to provide in vitro tissue/organ models and more effective therapies for curing tissue/organ failures.
AB - The fabrication of 3D tissues retaining the original functions of tissues/organs in vitro is crucial for optimal tissue engineering and regenerative medicine. The fabrication of 3D tissues also contributes to the establishment of in vitro tissue/organ models for drug screening. Our laboratory has developed a fabrication system for functional 3D tissues by stacking cell sheets of confluent cultured cells detached from a temperature-responsive culture dish. Here we describe the protocols for the fabrication of 3D tissues by cell sheet engineering. Three-dimensional cardiac tissues fabricated by stacking cardiac cell sheets pulsate spontaneously, synchronously and macroscopically. Via this protocol, it is also possible to fabricate other tissues, such as 3D tissue including capillary-like prevascular networks, from endothelial cells sandwiched between layered cell sheets. Cell sheet stacking technology promises to provide in vitro tissue/organ models and more effective therapies for curing tissue/organ failures.
UR - http://www.scopus.com/inward/record.url?scp=84859579818&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84859579818&partnerID=8YFLogxK
U2 - 10.1038/nprot.2012.027
DO - 10.1038/nprot.2012.027
M3 - Article
C2 - 22481530
AN - SCOPUS:84859579818
VL - 7
SP - 850
EP - 858
JO - Nature Protocols
JF - Nature Protocols
SN - 1754-2189
IS - 5
ER -