Fabrication of genetic diagnostic chip using DNA-arrayed TFT photosensor

Keiichi Hatakeyama, Tsuyoshi Tanaka, Masahiro Sawaguchi, Akihito Iwadate, Yasushi Mizutani, Kazuhiro Sasaki, Naofumi Tateishi, Tadashi Matsunaga

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This work describes a disposable DNA-arrayed thin film transistor (TFT) photosensor integrating a miniaturized reaction unit and a photoelectronic effect-based internal detection sensor. Single stranded DNA was chemically conjugated and arrayed on the TFT photosensor, which was fabricated by the semiconductor integrated circuit (IC) technology, for the detection of specific target DNA by DNA-DNA hybridization. The photosensor surface can be modified by various silane coupling reagents for the optimization of oligonucleotide immobilization conditions and single nucleotide polymorphism (SNP) detection was successfully employed using the TFT photosensor. A microfluidic channels were constructed on the TFT photosensor to improve the hybridization efficiency. The developed TFT photosensor will further promote the development of disposable photodetecting devices applicable in genetic diagnosis.

Original languageEnglish
Pages (from-to)573-575
Number of pages3
JournalElectrochemistry
Volume76
Issue number8
Publication statusPublished - 2008 Aug
Externally publishedYes

Keywords

  • Aldehyde dehydrogenase 2 (ALDH2) gene
  • Biochip
  • Single Nucleotide Polymorphism (SNP)
  • Thin Film Transistor (TFT) photosensor

ASJC Scopus subject areas

  • Electrochemistry

Fingerprint Dive into the research topics of 'Fabrication of genetic diagnostic chip using DNA-arrayed TFT photosensor'. Together they form a unique fingerprint.

  • Cite this

    Hatakeyama, K., Tanaka, T., Sawaguchi, M., Iwadate, A., Mizutani, Y., Sasaki, K., Tateishi, N., & Matsunaga, T. (2008). Fabrication of genetic diagnostic chip using DNA-arrayed TFT photosensor. Electrochemistry, 76(8), 573-575.