Fabrication of sensor for reactive oxygen species using gold electrodes modified with electropolymerized porphyrins and application for detection of stress of plants

Makoto Yuasa, Kenichi Oyaizu, Hidenori Murata, Tomohiro Kobayashi, Chihiro Kobayashi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Reactive Oxygen Spiecies (ROS) such as superoxide anion radical (.O(2)(-)) act as signals for the activation of stress-response and defense pathways. However, excess ROS generated by perturbing .O(2)(-) homeostasis stimulated many environmental stress, including intense light, drought, temperature stress, herbicides, induce high radical toxicity. Consequently, quantitative analysis of .O(2)(-) is a subject of intense research, since most of ROS are derived from .O(2)(-). Iron meso-tetrakis(3-thienyl)porphyrin complexes were electropolymerized onto a Au wire electrode. The modified Au electrode were applied to .O(2)(-) sensor to detect catalytic oxidation current of .O(2)(-) which was generated as an intermediate during the oxidation of xanthine by catalystic XOD. It was revealed that the sensor was quantitative to measure .O(2)(-). The modified Au electrode were applied to measure oxidation current of .O(2)(-) in mung beans under environmental stress condition. Plants were grown in atmosphere, 25 degrees C and in black darkness. The other plants were exposed to oxygen excess. The oxidation current of .O(2)(-) were increased plants were grown by high-oxygen environment compared to plants were grown at atmosphere. This experiment was indicated that environmental stress such as hyperoxia induced excess .O(2)(-) and Au wire sensor using iron porphyrin complexes is capable of .O(2)(-) detection in plants under environmental stresses.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalJournal of Oleo Science
Volume56
Issue number2
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

Porphyrins
Gold
Reactive Oxygen Species
Electrodes
Oxygen
Fabrication
Sensors
Atmosphere
Superoxides
Oxidation
Iron
Wire
Hyperoxia
Xanthine
Catalytic oxidation
Herbicides
Drought
Droughts
Darkness
Toxicity

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Fabrication of sensor for reactive oxygen species using gold electrodes modified with electropolymerized porphyrins and application for detection of stress of plants. / Yuasa, Makoto; Oyaizu, Kenichi; Murata, Hidenori; Kobayashi, Tomohiro; Kobayashi, Chihiro.

In: Journal of Oleo Science, Vol. 56, No. 2, 2007, p. 81-86.

Research output: Contribution to journalArticle

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AU - Kobayashi, Tomohiro

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