Abstract
In this paper, we present a method of fabricating a rigid antibody-immobilized surface using electric activation of a glutaraldehyde (GA)-modified aminopropylsilyl surface for stable antibody-modified field effect transistors (FETs). Electric activation of the GA-modified gate surface of the FET reduces Schiff bases, which are easily hydrolyzed and collapsed, formed between GA and 3-aminopropyltriethoxysilane, resulting in preventing the immobilized antibodies from desorbing from the surface. The lack of Raman peaks that could be assigned to a Schiff base after the electrical activation of the GA-modified surface indicated that the electric activation had reduced the Schiff base. The use of the antibody-modified FETs has three advantages for the detection of antigens: increased sensitivity, distinct recognition ability, and improved reproducibility. A tumor marker, alpha-fetoprotein (AFP), was quantitatively detected up to a concentration of 10. ng/mL using the antibody-modified FET. The detection ability of the FET accomplished a cutoff value of hepatic cancer. The quantitative detection of AFP in a solution with contaminating proteins was also demonstrated. This electric activation method is applicable to other antibody-modified FETs.
| Original language | English |
|---|---|
| Pages (from-to) | 2419-2425 |
| Number of pages | 7 |
| Journal | Biosensors and Bioelectronics |
| Volume | 26 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2011 Jan 15 |
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Keywords
- Antibody immobilization
- Field effect transistor
- Label-free detection
- Schiff base reduction
- Tumor marker
ASJC Scopus subject areas
- Biophysics
- Biomedical Engineering
- Biotechnology
- Electrochemistry
Cite this
Fabrication of stable antibody-modified field effect transistors using electrical activation of Schiff base cross-linkages for tumor marker detection. / Hideshima, Sho; Sato, Ryosuke; Kuroiwa, Shigeki; Osaka, Tetsuya.
In: Biosensors and Bioelectronics, Vol. 26, No. 5, 15.01.2011, p. 2419-2425.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fabrication of stable antibody-modified field effect transistors using electrical activation of Schiff base cross-linkages for tumor marker detection
AU - Hideshima, Sho
AU - Sato, Ryosuke
AU - Kuroiwa, Shigeki
AU - Osaka, Tetsuya
PY - 2011/1/15
Y1 - 2011/1/15
N2 - In this paper, we present a method of fabricating a rigid antibody-immobilized surface using electric activation of a glutaraldehyde (GA)-modified aminopropylsilyl surface for stable antibody-modified field effect transistors (FETs). Electric activation of the GA-modified gate surface of the FET reduces Schiff bases, which are easily hydrolyzed and collapsed, formed between GA and 3-aminopropyltriethoxysilane, resulting in preventing the immobilized antibodies from desorbing from the surface. The lack of Raman peaks that could be assigned to a Schiff base after the electrical activation of the GA-modified surface indicated that the electric activation had reduced the Schiff base. The use of the antibody-modified FETs has three advantages for the detection of antigens: increased sensitivity, distinct recognition ability, and improved reproducibility. A tumor marker, alpha-fetoprotein (AFP), was quantitatively detected up to a concentration of 10. ng/mL using the antibody-modified FET. The detection ability of the FET accomplished a cutoff value of hepatic cancer. The quantitative detection of AFP in a solution with contaminating proteins was also demonstrated. This electric activation method is applicable to other antibody-modified FETs.
AB - In this paper, we present a method of fabricating a rigid antibody-immobilized surface using electric activation of a glutaraldehyde (GA)-modified aminopropylsilyl surface for stable antibody-modified field effect transistors (FETs). Electric activation of the GA-modified gate surface of the FET reduces Schiff bases, which are easily hydrolyzed and collapsed, formed between GA and 3-aminopropyltriethoxysilane, resulting in preventing the immobilized antibodies from desorbing from the surface. The lack of Raman peaks that could be assigned to a Schiff base after the electrical activation of the GA-modified surface indicated that the electric activation had reduced the Schiff base. The use of the antibody-modified FETs has three advantages for the detection of antigens: increased sensitivity, distinct recognition ability, and improved reproducibility. A tumor marker, alpha-fetoprotein (AFP), was quantitatively detected up to a concentration of 10. ng/mL using the antibody-modified FET. The detection ability of the FET accomplished a cutoff value of hepatic cancer. The quantitative detection of AFP in a solution with contaminating proteins was also demonstrated. This electric activation method is applicable to other antibody-modified FETs.
KW - Antibody immobilization
KW - Field effect transistor
KW - Label-free detection
KW - Schiff base reduction
KW - Tumor marker
UR - http://www.scopus.com/inward/record.url?scp=78650606933&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650606933&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2010.10.023
DO - 10.1016/j.bios.2010.10.023
M3 - Article
C2 - 21074396
AN - SCOPUS:78650606933
VL - 26
SP - 2419
EP - 2425
JO - Biosensors
JF - Biosensors
SN - 0956-5663
IS - 5
ER -