Fabrication of thermo-responsive PNIPAAm-g-ETFE for cell culture dishes by pre-irradiation grafting

Yumi Yamahara, Naotsugu Nagasawa, Mitsumasa Taguchi, Akihiro Oshima, Masakazu Washio

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Thermo-responsive templates for the cell cultivation based on Poly(tetrafluoroethylene-co-ethylene) (ETFE) were fabricated by pre-irradiation grafting of N-isoproplyacrylamide (NIPAAm) monomer by electron beam (EB) irradiation under nitrogen gas atmosphere at room temperature, and their characteristic properties were studied. The detachment of cultured HeLa cells from fabricated thermo-responsive templates were attempted. Furthermore, the reaction mechanism is proposed using ESR spectroscopy and FT-IR spectroscopy.It is confirmed that the cultured HeLa cells were detached from fabricated thermo-responsive templates at 20°C. Water contact angle analysis indicated that obtained templates had thermo-response around 30°C. It is suggested that the grafted polymer chains would mainly react with peroxy radicals (-CF2-CF(OO[U+FF65])-) on tetrafluoroethylene unit in ETFE.

    Original languageEnglish
    JournalRadiation Physics and Chemistry
    DOIs
    Publication statusAccepted/In press - 2016 Oct 29

    Fingerprint

    parabolic reflectors
    ethylene
    templates
    fabrication
    irradiation
    cultured cells
    polytetrafluoroethylene
    detachment
    spectroscopy
    monomers
    electron beams
    nitrogen
    atmospheres
    polymers
    room temperature
    cells
    gases
    water

    Keywords

    • Cell culture dishes
    • ETFE
    • PNIPPAm-g-ETFE
    • Pre-irradiation grafting
    • Thermo-responsive

    ASJC Scopus subject areas

    • Radiation

    Cite this

    Fabrication of thermo-responsive PNIPAAm-g-ETFE for cell culture dishes by pre-irradiation grafting. / Yamahara, Yumi; Nagasawa, Naotsugu; Taguchi, Mitsumasa; Oshima, Akihiro; Washio, Masakazu.

    In: Radiation Physics and Chemistry, 29.10.2016.

    Research output: Contribution to journalArticle

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    abstract = "Thermo-responsive templates for the cell cultivation based on Poly(tetrafluoroethylene-co-ethylene) (ETFE) were fabricated by pre-irradiation grafting of N-isoproplyacrylamide (NIPAAm) monomer by electron beam (EB) irradiation under nitrogen gas atmosphere at room temperature, and their characteristic properties were studied. The detachment of cultured HeLa cells from fabricated thermo-responsive templates were attempted. Furthermore, the reaction mechanism is proposed using ESR spectroscopy and FT-IR spectroscopy.It is confirmed that the cultured HeLa cells were detached from fabricated thermo-responsive templates at 20°C. Water contact angle analysis indicated that obtained templates had thermo-response around 30°C. It is suggested that the grafted polymer chains would mainly react with peroxy radicals (-CF2-CF(OO[U+FF65])-) on tetrafluoroethylene unit in ETFE.",
    keywords = "Cell culture dishes, ETFE, PNIPPAm-g-ETFE, Pre-irradiation grafting, Thermo-responsive",
    author = "Yumi Yamahara and Naotsugu Nagasawa and Mitsumasa Taguchi and Akihiro Oshima and Masakazu Washio",
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    AU - Nagasawa, Naotsugu

    AU - Taguchi, Mitsumasa

    AU - Oshima, Akihiro

    AU - Washio, Masakazu

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    N2 - Thermo-responsive templates for the cell cultivation based on Poly(tetrafluoroethylene-co-ethylene) (ETFE) were fabricated by pre-irradiation grafting of N-isoproplyacrylamide (NIPAAm) monomer by electron beam (EB) irradiation under nitrogen gas atmosphere at room temperature, and their characteristic properties were studied. The detachment of cultured HeLa cells from fabricated thermo-responsive templates were attempted. Furthermore, the reaction mechanism is proposed using ESR spectroscopy and FT-IR spectroscopy.It is confirmed that the cultured HeLa cells were detached from fabricated thermo-responsive templates at 20°C. Water contact angle analysis indicated that obtained templates had thermo-response around 30°C. It is suggested that the grafted polymer chains would mainly react with peroxy radicals (-CF2-CF(OO[U+FF65])-) on tetrafluoroethylene unit in ETFE.

    AB - Thermo-responsive templates for the cell cultivation based on Poly(tetrafluoroethylene-co-ethylene) (ETFE) were fabricated by pre-irradiation grafting of N-isoproplyacrylamide (NIPAAm) monomer by electron beam (EB) irradiation under nitrogen gas atmosphere at room temperature, and their characteristic properties were studied. The detachment of cultured HeLa cells from fabricated thermo-responsive templates were attempted. Furthermore, the reaction mechanism is proposed using ESR spectroscopy and FT-IR spectroscopy.It is confirmed that the cultured HeLa cells were detached from fabricated thermo-responsive templates at 20°C. Water contact angle analysis indicated that obtained templates had thermo-response around 30°C. It is suggested that the grafted polymer chains would mainly react with peroxy radicals (-CF2-CF(OO[U+FF65])-) on tetrafluoroethylene unit in ETFE.

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