Fabrication of Three-Dimensional Tissues with Perfused Microchannels

Katsuhisa Sakaguchi, Tatsuya Shimizu, Kiyotaka Iwasaki, Masayuki Yamato, Mitsuo Umezu, Teruo Okano

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Recently, researchers have challenged to create three-dimensional (3-D) tissues with tissue engineering technology in order to establish in vitro models and new therapy for damaged organ. We have developed cell-sheet based tissue engineering and successfully fabricated pulsatile 3-D myocardial tissues both in vivo and in vitro by layering cardiac cell sheets. However, in vitro scaling up of 3-D cell-dense tissues is limited due to lack of blood vessels supplying oxygen and nutrition and removing waste molecules. In this study, we have developed novel bioreactor culturing layered cell sheets on collagen-based microchannels and examined cell behavior between tissues and channels. Rat cardiac cells including endothelial cells were cultured on temperature responsible culture dishes for 4 days. By lowering temperature, confluent cardiac cells were harvested as an intact cell sheet and two cardiac cell sheets are layered. Collagen-based microchannels were engineered by gelling collagen around parallel stainless wires and extracting the wires. The double-layer cell sheets were put on the microchannels and the constructs were connected to the novel perfusion bioreactor. After 5 days of cultivation, the tissue sections were stained with Hematoxylin-Eosin and endothelial cell specific Isolectin B4. HE staining demonstrated that layered cell sheets tightly connected onto the collagen microchannels. The microchannels maintained their patency during culture period. The cardiac cells migrated into collagen gel and the number of migration increased flow-rate dependently. At higher flow-rate, some cardiac cells reached to microchannels and covered over their inner surface. Isolectin B4 staining showed endothelial cells formed networks within the cell sheets and also played as migrating cells. We have successfully fabricated 3-D tissues with perfused microchannels and tissueoriginated cells migrated and communicated with the microchannels. These results showed new insights regarding in vitro vascular formation and indicated the possibility for fabricating vascularized 3-D tissues.

Original languageEnglish
Title of host publicationIFMBE Proceedings
Pages1213-1216
Number of pages4
Volume23
DOIs
Publication statusPublished - 2009
Event13th International Conference on Biomedical Engineering, ICBME 2008 -
Duration: 2008 Dec 32008 Dec 6

Other

Other13th International Conference on Biomedical Engineering, ICBME 2008
Period08/12/308/12/6

Fingerprint

Microchannels
Tissue
Fabrication
Collagen
Endothelial cells
Bioreactors
Tissue engineering
Lectins
Flow rate
Wire
Engineering technology
Blood vessels
Hematoxylin
Eosine Yellowish-(YS)
Nutrition
Cell culture
Rats
Gels
Oxygen
Temperature

Keywords

  • 3-D myocyte tissues
  • Bioreactor
  • Cell sheet
  • Tissue engineering
  • Vascular formation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Fabrication of Three-Dimensional Tissues with Perfused Microchannels. / Sakaguchi, Katsuhisa; Shimizu, Tatsuya; Iwasaki, Kiyotaka; Yamato, Masayuki; Umezu, Mitsuo; Okano, Teruo.

IFMBE Proceedings. Vol. 23 2009. p. 1213-1216.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sakaguchi, K, Shimizu, T, Iwasaki, K, Yamato, M, Umezu, M & Okano, T 2009, Fabrication of Three-Dimensional Tissues with Perfused Microchannels. in IFMBE Proceedings. vol. 23, pp. 1213-1216, 13th International Conference on Biomedical Engineering, ICBME 2008, 08/12/3. https://doi.org/10.1007/978-3-540-92841-6_297
Sakaguchi, Katsuhisa ; Shimizu, Tatsuya ; Iwasaki, Kiyotaka ; Yamato, Masayuki ; Umezu, Mitsuo ; Okano, Teruo. / Fabrication of Three-Dimensional Tissues with Perfused Microchannels. IFMBE Proceedings. Vol. 23 2009. pp. 1213-1216
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