Fabrication process of micro-patterns on cell-culturing substrates toward high-resolution control and observation of cell migration

Michiko Sugawara, Wonjun Choi, Jun Nakanishi, Kazuo Yamaguchi, Hideo Yokota, Tohru Yagi

Research output: Contribution to journalArticle

1 Citation (Scopus)


It is well known that cell migrations play a key role in the living systems. From the basic technical point of view, cell migration control is one of the important and useful ways to clarify the mechanism of the cell migration qualitatively and quantitatively. For the purpose of high-resolution control and observation of the cell migrations, which is the goal of this research, in this study, an attempt was made to establish the fabrication process of micro-patterns on caged cell-culturing substrates using inverted microscope with high magnification objective lens. With the combination of fluorescence antibody technique, the adequate ultraviolet (UV) lay exposure time was obtained for the formation of micro-patterns of fibronectin on the caged cell-culturing substrates. We also confirmed that it was possible to control the position of micro-patterns by the primary and secondary UV exposure. In addition, Swiss 3T3 cell culture experiment demonstrated that the initial shape of the cell could be restricted by the primary UV exposure and that the secondary UV exposure was remarkably useful to control the cell migration.

Original languageEnglish
Pages (from-to)833-839
Number of pages7
JournalIEEJ Transactions on Electronics, Information and Systems
Issue number4
Publication statusPublished - 2011 Jan 1
Externally publishedYes



  • Caged cell-culturing substrates
  • Cell migration control
  • Fluorescence antibody technique
  • Reduction projection lithography

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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