Facile preparation of photoactivatable surfaces with tuned substrate adhesiveness

Yoshihisa Shimizu, Masao Kamimura, Shota Yamamoto, Shimaa A. Abdellatef, Kazuo Yamaguchi, Jun Nakanishi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This paper describes a facile method for the preparation of photoactivatable substrates with tuned surface density of an extracellular matrix peptide to resolve the impacts of biochemical and mechanical cues on collective cell migration. The controllability of surface ligand density was validated by cell adhesion and migration tests, complemented with fluorescence observation of an alternative ligand. Depending on the surface ligand density, HeLa cells either kept or lost collective characteristics. The present materials will be useful to address mechanobiology of collective cell migration.

Original languageEnglish
Pages (from-to)1183-1188
Number of pages6
JournalAnalytical Sciences
Volume32
Issue number11
DOIs
Publication statusPublished - 2016 Jan 1
Externally publishedYes

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Keywords

  • Cell adhesion
  • Cell migration
  • Focal adhesion
  • Mechanics
  • Mechanobiology
  • Photochemical reaction
  • Self-assembled monolayer

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Shimizu, Y., Kamimura, M., Yamamoto, S., Abdellatef, S. A., Yamaguchi, K., & Nakanishi, J. (2016). Facile preparation of photoactivatable surfaces with tuned substrate adhesiveness. Analytical Sciences, 32(11), 1183-1188. https://doi.org/10.2116/analsci.32.1183