Facile preparation of single-cell arrays on caged cell-culturing substrates

Yukiko Kikuchi, Jun Nakanishi, Tohru Takarada, Satoshi Inoue, Kazuo Yamaguchi, Yasuhiko Yoshida, Mizuo Maeda

Research output: Contribution to conferencePaperpeer-review

Abstract

Caged cell-culturing substrate is composed of a glass substrate immobilized with an alkylsiloxane monolayer having a photocleavable protecting group. This substrate allows to form cell-adhesive regions by UV illumination under a conventional fluorescence microscope. In the present study, we examined the effect of terminal functional groups on the efficiencies of photoactivation for cell adhesion. Among four tested substrates terminating with different functional groups, the photoactivation of cell adhesion was the most effective on that terminating with an amino group. In addition, we demonstrated a facile preparation of single-cell arrays on this substrate.

Original languageEnglish
Pages5428-5429
Number of pages2
Publication statusPublished - 2006
Event55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
Duration: 2006 Sep 202006 Sep 22

Other

Other55th Society of Polymer Science Japan Symposium on Macromolecules
CountryJapan
CityToyama
Period06/9/2006/9/22

Keywords

  • Caged compound
  • Cell adhesion
  • Cell array
  • Functional group

ASJC Scopus subject areas

  • Engineering(all)

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