Facilely fabricated luminescent nanoparticle thermosensor for real-time microthermography in living animals

Ferdinandus, Satoshi Arai, Shinji Takeoka, Shinichi Ishiwata, Madoka Suzuki, Hirotaka Sato

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This paper presents a high-sensitivity luminescent nanoparticle thermosensor capable of real-time microthermography in a living organism. Microthermography, or microscopically visualizing the temperature distribution within living cells, tissues, and organisms, is a promising technology to explore various physiological activities at the microscale. Using a facile nanoprecipitation method, we fabricated a polymer-nanoparticle embedding EuDT, a thermosensitive high-luminescence-emitter dye molecule, and rhodamine 800, a luminescent molecule excitable with low energy light and less sensitive to temperature. The nanoparticle thermosensor was largely exempted from the background noise, which is the undesired luminescence from the target biological sample, enabling direct acquisition of luminescence intensities from the thermosensor within the specified area of 68 μm × 68 μm on the muscle tissue of a living insect, i.e., real-time microthermography, without the need of subtracting background noise. Thus, we successfully mapped out the temperature shift due to the animal's voluntary heat production. The nanoparticle thermosensor capable of in vivo temperature mapping must be a useful biological thermographic technology to explore microscopic heat productions in living organisms.

Original languageEnglish
Pages (from-to)1222-1227
Number of pages6
JournalACS Sensors
Volume1
Issue number10
DOIs
Publication statusPublished - 2016 Oct 1

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animals
Animals
organisms
Nanoparticles
Luminescence
nanoparticles
background noise
luminescence
Tissue
heat
Molecules
insects
rhodamine
muscles
microbalances
Temperature
embedding
temperature
Muscle
molecules

Keywords

  • Bioimaging
  • Fluorescent thermosensor
  • In vivo thermography
  • Luminescent thermosensor
  • Nanoparticle thermosensor
  • Temperature sensitive dye
  • Thermogenesis
  • Thermography

ASJC Scopus subject areas

  • Bioengineering
  • Fluid Flow and Transfer Processes
  • Process Chemistry and Technology
  • Instrumentation

Cite this

Facilely fabricated luminescent nanoparticle thermosensor for real-time microthermography in living animals. / Ferdinandus; Arai, Satoshi; Takeoka, Shinji; Ishiwata, Shinichi; Suzuki, Madoka; Sato, Hirotaka.

In: ACS Sensors, Vol. 1, No. 10, 01.10.2016, p. 1222-1227.

Research output: Contribution to journalArticle

Ferdinandus ; Arai, Satoshi ; Takeoka, Shinji ; Ishiwata, Shinichi ; Suzuki, Madoka ; Sato, Hirotaka. / Facilely fabricated luminescent nanoparticle thermosensor for real-time microthermography in living animals. In: ACS Sensors. 2016 ; Vol. 1, No. 10. pp. 1222-1227.
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