Facilitation of α-amino-3-hydroxy-5-methylisoxazole-4-propionate receptor transmission in the suprachiasmatic nucleus by aniracetam enhances photic responses of the biological clock in rodents

Takahiro Moriya, Masayuki Ikeda, Koji Teshima, Reiko Hara, Koji Kuriyama, Tohru Yoshioka, Charles N. Allent, Shigenobu Shibata

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

This study was designed to test whether the α-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptor facilitating drug, aniracetam, could potentiate photic responses of the biological clock in the suprachiasmatic nucleus (SCN) of rodents. Using the whole-cell patch technique, we first demonstrated that AMPA currents elicited by either local AMPA application or optic chiasm stimulation were augmented by aniracetam in the neurons of the SCN. The AMPA application-elicited increase of intracellular Ca2+ concentration in SCN slices was also enhanced by aniracetam treatment. The systemic injection of aniracetam dosedependently (10-100 mg/kg) potentiated the phase delay in behavioral rhythm induced by brief light exposure of low intensity (3 lux) but not high intensity (10 or 60 lux) during early subjective night. Under the blockade of NMDA receptors by (+) MK801, aniracetam failed to potentiate a light (3 lux) induced phase delay in behavioral rhythm. Aniracetam increased the photic induction of c-Fos protein in the SCN that was elicited by low intensity light exposure (3 lux). These results suggest that AMPA receptor-mediated responses facilitated by aniracetam can explain enhanced photic responses of the biological clock in the SCN of rodents.

Original languageEnglish
Pages (from-to)978-987
Number of pages10
JournalJournal of neurochemistry
Volume85
Issue number4
DOIs
Publication statusPublished - 2003 May

Keywords

  • Behavioral rhythm
  • Ca imaging
  • Circadian rhythm
  • Whole-cell patch clamp
  • c-Fos protein

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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