Falsirhodobacter sp. alg1 Harbors Single Homologs of Endo and Exo-Type Alginate Lyases Efficient for Alginate Depolymerization

Tetsushi Mori, Mami Takahashi, Reiji Tanaka, Hideo Miyake, Toshiyuki Shibata, Seinen Chow, Kouichi Kuroda, Mitsuyoshi Ueda, Haruko Takeyama

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Alginate-degrading bacteria play an important role in alginate degradation by harboring highly efficient and unique alginolytic genes. Although the general mechanism for alginate degradation by these bacteria is fairly understood, much is still required to fully exploit them. Here, we report the isolation of a novel strain, Falsirhodobacter sp. alg1, the first report for an alginate-degrading bacterium from the family Rhodobacteraceae. Genome sequencing reveals that strain alg1 harbors a primary alginate degradation pathway with only single homologs of an endo- and exo-type alginate lyase, AlyFRA and AlyFRB, which is uncommon among such bacteria. Subsequent functional analysis showed that both enzymes were extremely efficient to depolymerize alginate suggesting evolutionary interests in the acquirement of these enzymes. The exo-type alginate lyase, AlyFRB in particular could depolymerize alginate without producing intermediate products making it a highly efficient enzyme for the production of 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH). Based on our findings, we believe that the discovery of Falsirhodobacter sp. alg1 and its alginolytic genes hints at the potentiality of a more diverse and unique population of alginate-degrading bacteria.

    Original languageEnglish
    Article numbere0155537
    JournalPLoS One
    Volume11
    Issue number5
    DOIs
    Publication statusPublished - 2016 May 1

    Fingerprint

    Depolymerization
    depolymerization
    alginates
    Ports and harbors
    Bacteria
    bacteria
    Genes
    Degradation
    Rhodobacteraceae
    degradation
    Enzymes
    enzymes
    Uronic Acids
    Functional analysis
    uronic acids
    poly(beta-D-mannuronate) lyase
    alginic acid
    alginate lyase
    genes
    Genome

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Falsirhodobacter sp. alg1 Harbors Single Homologs of Endo and Exo-Type Alginate Lyases Efficient for Alginate Depolymerization. / Mori, Tetsushi; Takahashi, Mami; Tanaka, Reiji; Miyake, Hideo; Shibata, Toshiyuki; Chow, Seinen; Kuroda, Kouichi; Ueda, Mitsuyoshi; Takeyama, Haruko.

    In: PLoS One, Vol. 11, No. 5, e0155537, 01.05.2016.

    Research output: Contribution to journalArticle

    Mori, Tetsushi ; Takahashi, Mami ; Tanaka, Reiji ; Miyake, Hideo ; Shibata, Toshiyuki ; Chow, Seinen ; Kuroda, Kouichi ; Ueda, Mitsuyoshi ; Takeyama, Haruko. / Falsirhodobacter sp. alg1 Harbors Single Homologs of Endo and Exo-Type Alginate Lyases Efficient for Alginate Depolymerization. In: PLoS One. 2016 ; Vol. 11, No. 5.
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