Fast analysis of biological compounds by gradient liquid chromatography using pillar array column

Yanting Song, Masao Noguchi, Katsuya Takatsuki, Tetsushi Sekiguchi, Jun Mizuno, Takashi Funatsu, Shuichi Shoji, Makoto Tsunoda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Here we describe fast analysis of biomolecules by newly developed pressure-driven liquid chromatography (LC) on a chip with integration of a gradient elution system. We have reported the fabrication of a gradient elution system using a cross-Tesla mixer on a chip with pillar array columns [1]. In this study, we applied the chip to the analysis of biological compounds, and showed that 10 times faster separation was achieved under gradient elution than under isocratic elution. The retention times under gradient elution conditions were corresponded to the values estimated by semi-empirical equation, which showed that the gradient elution system worked validly.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages587-589
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa
Duration: 2012 Oct 282012 Nov 1

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CityOkinawa
Period12/10/2812/11/1

Fingerprint

Liquid chromatography
Biomolecules
Fabrication

Keywords

  • Aliphatic amines
  • Fluorescence
  • NBD-F
  • Peak width
  • Pressure driven

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Song, Y., Noguchi, M., Takatsuki, K., Sekiguchi, T., Mizuno, J., Funatsu, T., ... Tsunoda, M. (2012). Fast analysis of biological compounds by gradient liquid chromatography using pillar array column. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 587-589). Chemical and Biological Microsystems Society.

Fast analysis of biological compounds by gradient liquid chromatography using pillar array column. / Song, Yanting; Noguchi, Masao; Takatsuki, Katsuya; Sekiguchi, Tetsushi; Mizuno, Jun; Funatsu, Takashi; Shoji, Shuichi; Tsunoda, Makoto.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 587-589.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Song, Y, Noguchi, M, Takatsuki, K, Sekiguchi, T, Mizuno, J, Funatsu, T, Shoji, S & Tsunoda, M 2012, Fast analysis of biological compounds by gradient liquid chromatography using pillar array column. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 587-589, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, 12/10/28.
Song Y, Noguchi M, Takatsuki K, Sekiguchi T, Mizuno J, Funatsu T et al. Fast analysis of biological compounds by gradient liquid chromatography using pillar array column. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 587-589
Song, Yanting ; Noguchi, Masao ; Takatsuki, Katsuya ; Sekiguchi, Tetsushi ; Mizuno, Jun ; Funatsu, Takashi ; Shoji, Shuichi ; Tsunoda, Makoto. / Fast analysis of biological compounds by gradient liquid chromatography using pillar array column. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 587-589
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