Fatty acid and lipid composition of an eicosapentaenoic acid-producing marine bacterium

Kazuo Watanabe, Chikako Ishikawa, Kazunaga Yazawa, Kiyosi Kondo, Akihiko Kawaguchi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The major lipids of Shewanella sp. strain SCRC-2738, which produces large amounts of eicosapentaenoic acid (ERA), 20:5(n-3), were phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). These phospholipids comprised the same fatty acid components. The ratio of 20:5 (n-3) to total fatty acids in PG was about threefold greater than in PE. In both phospholipids, 20:5(n-3) was exclusively localized at the sn-2 position. Productivity of 20:5(n-3) was dependent on the growth temperature of the bacterium; it did not vary between 10 and 20°C, but decreased to about 85% at 25°C, declined to half at 27°C, and to below the detection limit at 30°C. Although 14C-labeled exogenous palmitate, 16:0, oleate, 18:1(n-9), linoleate, 18:2(n-6), and eicosatrienoate, 20:3(n-6) were incorporated into the phospholipids, they were not further metabolized. The desaturation mechanism to 20:5(n-3) of this bacterium seems to be different from those of higher plants in that the lipid-bound fatty acyl chain is used as a substrate for desaturation.

Original languageEnglish
Pages (from-to)104-112
Number of pages9
JournalJournal of Marine Biotechnology
Volume4
Issue number2
Publication statusPublished - 1996
Externally publishedYes

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Eicosapentaenoic Acid
Phospholipids
Bacteria
Phosphatidylglycerols
Fatty Acids
Lipids
Chemical analysis
Shewanella
Palmitates
Growth temperature
Linoleic Acid
Oleic Acid
Limit of Detection
Productivity
Temperature
Substrates
Growth
phosphatidylethanolamine

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Watanabe, K., Ishikawa, C., Yazawa, K., Kondo, K., & Kawaguchi, A. (1996). Fatty acid and lipid composition of an eicosapentaenoic acid-producing marine bacterium. Journal of Marine Biotechnology, 4(2), 104-112.

Fatty acid and lipid composition of an eicosapentaenoic acid-producing marine bacterium. / Watanabe, Kazuo; Ishikawa, Chikako; Yazawa, Kazunaga; Kondo, Kiyosi; Kawaguchi, Akihiko.

In: Journal of Marine Biotechnology, Vol. 4, No. 2, 1996, p. 104-112.

Research output: Contribution to journalArticle

Watanabe, K, Ishikawa, C, Yazawa, K, Kondo, K & Kawaguchi, A 1996, 'Fatty acid and lipid composition of an eicosapentaenoic acid-producing marine bacterium', Journal of Marine Biotechnology, vol. 4, no. 2, pp. 104-112.
Watanabe, Kazuo ; Ishikawa, Chikako ; Yazawa, Kazunaga ; Kondo, Kiyosi ; Kawaguchi, Akihiko. / Fatty acid and lipid composition of an eicosapentaenoic acid-producing marine bacterium. In: Journal of Marine Biotechnology. 1996 ; Vol. 4, No. 2. pp. 104-112.
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