Fe deficiency induces phosphorylation and translocation of Lhcb1 in barley thylakoid membranes

Akihiro Saito, Mizuho Shimizu, Hitomi Nakamura, Shoko Maeno, Riko Katase, Eitaro Miwa, Kyoko Higuchi, Kintake Sonoike

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    9 Citations (Scopus)

    Abstract

    HvLhcb1 a major light-harvesting chlorophyll a/b-binding protein in barley, is a critical player in sustainable growth under Fe deficiency. Here, we demonstrate that Fe deficiency induces phosphorylation of HvLhcb1 proteins leading to their migration from grana stacks to stroma thylakoid membranes. HvLhcb1 remained phosphorylated even in the dark and apparently independently of state transition, which represents a mechanism for short-term acclimation. Our data suggest that the constitutive phosphorylation-triggered translocation of HvLhcb1 under Fe deficiency contributes to optimization of the excitation balance between photosystem II and photosystem I, the latter of which is a main target of Fe deficiency.

    Original languageEnglish
    Pages (from-to)2042-2048
    Number of pages7
    JournalFEBS Letters
    Volume588
    Issue number12
    DOIs
    Publication statusPublished - 2014 Jul 5

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    Keywords

    • Barley
    • Iron deficiency
    • Light-harvesting antenna complex II
    • Non-photochemical quenching
    • Photosynthetic electron transport
    • Protein phosphorylation

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Cell Biology
    • Genetics
    • Molecular Biology
    • Structural Biology

    Cite this

    Saito, A., Shimizu, M., Nakamura, H., Maeno, S., Katase, R., Miwa, E., Higuchi, K., & Sonoike, K. (2014). Fe deficiency induces phosphorylation and translocation of Lhcb1 in barley thylakoid membranes. FEBS Letters, 588(12), 2042-2048. https://doi.org/10.1016/j.febslet.2014.04.031