Fecal excretion of orally administered collagen-like peptides in rats: Contribution of the triple-helical conformation to their stability

Takaki Koide, Naoyuki Yamamoto, Kazuma B. Taira, Hiroyuki Yasui

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Orally ingested peptides are generally digested in the gastrointestinal (GI) tract and absorbed in the form of oligopeptides. We previously reported that intravenously administered collagen-like triple-helical peptides circulated in the bloodstream and were excreted in their intact forms in urine nearly quantitatively. In the present study, we investigated the fates of orally administered collagen-like peptides in rats. (Pro-Hyp-Gly)10 (Hyp: 4-hydroxyproline), which formed a stable triple-helical structure, was stable in the GI tract, and 72.3±13.0% of the peptide was excreted in the feces. Its recovery ratio was similar to that of all-D-(Pro-Pro-Gly)10 (75.1±15.7%), the indigestible control. In contrast, (Pro-Hyp-Gly)5 and (Pro-Pro-Gly)10, the random coil conformations of which were dominant at body temperature, were not detected in fecal samples, indicating that they were digested by proteases. The high stability of the triple-helical conformation in mammalian bodies suggests the potential use of collagen-like peptides as novel scaffolds of peptide drugs.

    Original languageEnglish
    Pages (from-to)135-137
    Number of pages3
    JournalBiological and Pharmaceutical Bulletin
    Volume39
    Issue number1
    Publication statusPublished - 2016 Jan 1

    Keywords

    • Collagen
    • Digestion
    • Feces
    • Peptide
    • Triple helix

    ASJC Scopus subject areas

    • Pharmaceutical Science
    • Pharmacology

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