Fiber-optic sensor with a sandwich binding technique for fluoroimmunoassay

Noriyuki Nakamura, Tadashi Matsunaga

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We report the development of a fiber-optic sensor based on the principle of the sandwich binding technique for the fluoro-immunoassay. Anti-mouse immunoglobulin G (IgG) antibody was immobilized on the membrane mounted to the edge of the fiber. The sensor was exposed to the solution containing mouse IgG and then allophycocyanin conjugated anti-mouse IgG antibody was added. The helium-neon (He-Ne) laser provides excitation of the sandwich binding antigen-antibody complex. This results in fluorescence emission at the membrane. Increase in the fluorescence intensity from sensing tip was proportional to the amount of mouse IgG in the sample. A linear relationship was obtained betweenthe fluorescence intensity increase and the mouse IgG concentration in the range 0.3 - 9.0 pg/ml.

Original languageEnglish
Pages (from-to)1075-1084
Number of pages10
JournalAnalytical Letters
Volume24
Issue number7
DOIs
Publication statusPublished - 1991 Jul 1
Externally publishedYes

Keywords

  • Fiber-Optic Sensor
  • Fluoroimmunoassay
  • Immunoglobulin G
  • Sandwich Binding Technique

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry, medical
  • Clinical Biochemistry
  • Biochemistry

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