Fibrinogen-conjugated albumin polymers and their interaction with platelets under flow conditions

Shinji Takeoka, Y. Teramura, Y. Okamura, M. Handa, Y. Ikeda, E. Tsuchida

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    Albumin polymers, having an average diameter of 1020 ± 250 nm, were prepared by the disulfide polymerization of recombinant human serum albumin (rHSA) by controlling of the pH and temperature. Fibrinogen could be conjugated on the surface of an albumin polymer using N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP). Under flow conditions, the fibrinogen-conjugated albumin polymers (fibrinogen-albumin polymers) were irreversibly attached to the platelet-immobilized surface in the reconstituted blood at a low platelet concentration ([platelet] = 5.0 × 104/μL, a 5-fold diluted platelet concentration), and the attachment was suppressed by the addition of anti-GPIIb/IIIa monoclonal antibodies. It was confirmed that fibrinogen-albumin polymers specifically interacted with GPIIb/IIIa expressed on the surface of the activated platelets. Although platelets with a low platelet concentration were hardly attached to the platelet-immobilized surface under the flow conditions, the addition of fibrinogen-albumin polymers enhanced the attachment of the remaining platelets to the surface, indicating that the fibrinogen-albumin polymers would help the hemostatic ability of platelets at the site of vascular injury of patients in thrombocytopenia.

    Original languageEnglish
    Pages (from-to)1192-1197
    Number of pages6
    JournalBiomacromolecules
    Volume2
    Issue number4
    DOIs
    Publication statusPublished - 2001

    Fingerprint

    Conjugated polymers
    Platelets
    Fibrinogen
    Albumins
    Polymers
    Blood Platelets
    Monoclonal antibodies
    Vascular System Injuries
    Hemostatics
    Serum Albumin
    Polymerization
    Disulfides
    Thrombocytopenia
    Blood
    Monoclonal Antibodies
    Temperature

    ASJC Scopus subject areas

    • Organic Chemistry
    • Biochemistry, Genetics and Molecular Biology(all)
    • Polymers and Plastics
    • Materials Chemistry

    Cite this

    Fibrinogen-conjugated albumin polymers and their interaction with platelets under flow conditions. / Takeoka, Shinji; Teramura, Y.; Okamura, Y.; Handa, M.; Ikeda, Y.; Tsuchida, E.

    In: Biomacromolecules, Vol. 2, No. 4, 2001, p. 1192-1197.

    Research output: Contribution to journalArticle

    Takeoka, Shinji ; Teramura, Y. ; Okamura, Y. ; Handa, M. ; Ikeda, Y. ; Tsuchida, E. / Fibrinogen-conjugated albumin polymers and their interaction with platelets under flow conditions. In: Biomacromolecules. 2001 ; Vol. 2, No. 4. pp. 1192-1197.
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    AU - Handa, M.

    AU - Ikeda, Y.

    AU - Tsuchida, E.

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