Fibronectin binding is required for acquisition of mesenchymal/endothelial differentiation potential in human circulating monocytes

Noriyuki Seta, Yuka Okazaki, Keisuke Izumi, Hiroshi Miyazaki, Takashi Kato, Masataka Kuwana

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    We previously reported monocyte-derived multipotential cells (MOMCs), which include progenitors capable of differentiating into a variety of mesenchymal cells and endothelial cells. In vitro generation of MOMCs from circulating CD14+ monocytes requires their binding to extracellular matrix (ECM) protein and exposure to soluble factor(s) derived from circulating CD14 - cells. Here, we investigated the molecular factors involved in MOMC generation by examining the binding of monocytes to ECM proteins. We found that MOMCs were obtained on the fibronectin, but not on type I collagen, laminin, or poly-L-lysine. MOMC generation was followed by changes in the expression profiles of transcription factors and was completely inhibited by either anti-α5 integrin antibody or a synthetic peptide that competed with the RGD domain for the β1-integrin binding site. These results indicate that acquisition of the multidifferentiation potential by circulating monocytes depends on their binding to the RGD domain of fibronectin via cell-surface α5β1 integrin.

    Original languageEnglish
    Article number820827
    JournalClinical and Developmental Immunology
    Volume2012
    DOIs
    Publication statusPublished - 2012

    Fingerprint

    Fibronectins
    Monocytes
    Integrins
    Extracellular Matrix Proteins
    Laminin
    Collagen Type I
    Lysine
    Transcription Factors
    Endothelial Cells
    Binding Sites
    Peptides
    Antibodies

    ASJC Scopus subject areas

    • Immunology
    • Immunology and Allergy
    • Medicine(all)

    Cite this

    Fibronectin binding is required for acquisition of mesenchymal/endothelial differentiation potential in human circulating monocytes. / Seta, Noriyuki; Okazaki, Yuka; Izumi, Keisuke; Miyazaki, Hiroshi; Kato, Takashi; Kuwana, Masataka.

    In: Clinical and Developmental Immunology, Vol. 2012, 820827, 2012.

    Research output: Contribution to journalArticle

    @article{01a550c82afe4bd4bbc82942c3149914,
    title = "Fibronectin binding is required for acquisition of mesenchymal/endothelial differentiation potential in human circulating monocytes",
    abstract = "We previously reported monocyte-derived multipotential cells (MOMCs), which include progenitors capable of differentiating into a variety of mesenchymal cells and endothelial cells. In vitro generation of MOMCs from circulating CD14+ monocytes requires their binding to extracellular matrix (ECM) protein and exposure to soluble factor(s) derived from circulating CD14 - cells. Here, we investigated the molecular factors involved in MOMC generation by examining the binding of monocytes to ECM proteins. We found that MOMCs were obtained on the fibronectin, but not on type I collagen, laminin, or poly-L-lysine. MOMC generation was followed by changes in the expression profiles of transcription factors and was completely inhibited by either anti-α5 integrin antibody or a synthetic peptide that competed with the RGD domain for the β1-integrin binding site. These results indicate that acquisition of the multidifferentiation potential by circulating monocytes depends on their binding to the RGD domain of fibronectin via cell-surface α5β1 integrin.",
    author = "Noriyuki Seta and Yuka Okazaki and Keisuke Izumi and Hiroshi Miyazaki and Takashi Kato and Masataka Kuwana",
    year = "2012",
    doi = "10.1155/2012/820827",
    language = "English",
    volume = "2012",
    journal = "Journal of Immunology Research",
    issn = "2314-8861",
    publisher = "Hindawi Publishing Corporation",

    }

    TY - JOUR

    T1 - Fibronectin binding is required for acquisition of mesenchymal/endothelial differentiation potential in human circulating monocytes

    AU - Seta, Noriyuki

    AU - Okazaki, Yuka

    AU - Izumi, Keisuke

    AU - Miyazaki, Hiroshi

    AU - Kato, Takashi

    AU - Kuwana, Masataka

    PY - 2012

    Y1 - 2012

    N2 - We previously reported monocyte-derived multipotential cells (MOMCs), which include progenitors capable of differentiating into a variety of mesenchymal cells and endothelial cells. In vitro generation of MOMCs from circulating CD14+ monocytes requires their binding to extracellular matrix (ECM) protein and exposure to soluble factor(s) derived from circulating CD14 - cells. Here, we investigated the molecular factors involved in MOMC generation by examining the binding of monocytes to ECM proteins. We found that MOMCs were obtained on the fibronectin, but not on type I collagen, laminin, or poly-L-lysine. MOMC generation was followed by changes in the expression profiles of transcription factors and was completely inhibited by either anti-α5 integrin antibody or a synthetic peptide that competed with the RGD domain for the β1-integrin binding site. These results indicate that acquisition of the multidifferentiation potential by circulating monocytes depends on their binding to the RGD domain of fibronectin via cell-surface α5β1 integrin.

    AB - We previously reported monocyte-derived multipotential cells (MOMCs), which include progenitors capable of differentiating into a variety of mesenchymal cells and endothelial cells. In vitro generation of MOMCs from circulating CD14+ monocytes requires their binding to extracellular matrix (ECM) protein and exposure to soluble factor(s) derived from circulating CD14 - cells. Here, we investigated the molecular factors involved in MOMC generation by examining the binding of monocytes to ECM proteins. We found that MOMCs were obtained on the fibronectin, but not on type I collagen, laminin, or poly-L-lysine. MOMC generation was followed by changes in the expression profiles of transcription factors and was completely inhibited by either anti-α5 integrin antibody or a synthetic peptide that competed with the RGD domain for the β1-integrin binding site. These results indicate that acquisition of the multidifferentiation potential by circulating monocytes depends on their binding to the RGD domain of fibronectin via cell-surface α5β1 integrin.

    UR - http://www.scopus.com/inward/record.url?scp=84870172404&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84870172404&partnerID=8YFLogxK

    U2 - 10.1155/2012/820827

    DO - 10.1155/2012/820827

    M3 - Article

    VL - 2012

    JO - Journal of Immunology Research

    JF - Journal of Immunology Research

    SN - 2314-8861

    M1 - 820827

    ER -