Fibronectin binding is required for acquisition of mesenchymal/endothelial differentiation potential in human circulating monocytes

Noriyuki Seta, Yuka Okazaki, Keisuke Izumi, Hiroshi Miyazaki, Takashi Kato, Masataka Kuwana

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

We previously reported monocyte-derived multipotential cells (MOMCs), which include progenitors capable of differentiating into a variety of mesenchymal cells and endothelial cells. In vitro generation of MOMCs from circulating CD14+ monocytes requires their binding to extracellular matrix (ECM) protein and exposure to soluble factor(s) derived from circulating CD14 - cells. Here, we investigated the molecular factors involved in MOMC generation by examining the binding of monocytes to ECM proteins. We found that MOMCs were obtained on the fibronectin, but not on type I collagen, laminin, or poly-L-lysine. MOMC generation was followed by changes in the expression profiles of transcription factors and was completely inhibited by either anti-α5 integrin antibody or a synthetic peptide that competed with the RGD domain for the β1-integrin binding site. These results indicate that acquisition of the multidifferentiation potential by circulating monocytes depends on their binding to the RGD domain of fibronectin via cell-surface α5β1 integrin.

Original languageEnglish
Article number820827
JournalClinical and Developmental Immunology
Volume2012
DOIs
Publication statusPublished - 2012

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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