Fibronectin-calcium phosphate composite layer on hydroxyapatite to enhance adhesion, cell spread and osteogenic differentiation of human mesenchymal stem cells in vitro

Yu Sogo, Atsuo Ito, Tomonori Matsuno, Ayako Oyane, Gaku Tamazawa, Tazuko Satoh, Atsushi Yamazaki, Eiji Uchimura, Tadao Ohno

    Research output: Contribution to journalArticle

    36 Citations (Scopus)

    Abstract

    Fibronectin (Fn) and type I collagen (Col) were immobilized on a surface of a hydroxyapatite (HAP) ceramic by coprecipitation with calcium phosphate in a supersaturated calcium phosphate solution prepared by mixing clinically approved infusion fluids. These proteins and the calcium phosphate precipitate formed a composite surface layer. As a result, the proteins were immobilized firmly as not to be released completely for 3 d in a physiological salt solution. When human mesenchymal stem cells (hMSCs) were cultured on a HAP ceramic in a differentiation medium supplemented with dexamethasone, β-glycerophosphate and ascorbic acid, hMSCs spread well within 1 h. The alkaline phosphatase (ALP) activity of hMSCs cultured on the Fn-calcium phosphate composite layer significantly increased compared with that of hMSCs cultured on the untreated HAP ceramic. On the other hand, Col did not increase the ALP activity of hMSCs and no synergy between Fn and Col was observed. Therefore, the Fn-calcium phosphate composite layer formed on the HAP is useful for the enhancement of the spreading and osteogenic differentiation of hMSCs in vitro.

    Original languageEnglish
    Article number009
    Pages (from-to)116-123
    Number of pages8
    JournalBiomedical Materials
    Volume2
    Issue number2
    DOIs
    Publication statusPublished - 2007 Jun 1

    Fingerprint

    Cell adhesion
    Calcium phosphate
    Durapatite
    Stem cells
    Mesenchymal Stromal Cells
    Hydroxyapatite
    Fibronectins
    Cell Adhesion
    Composite materials
    Ceramics
    Collagen
    Phosphatases
    Human Activities
    Alkaline Phosphatase
    Immobilized Proteins
    Glycerophosphates
    Proteins
    Ascorbic acid
    Coprecipitation
    Collagen Type I

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Biomedical Engineering
    • Biophysics
    • Biomaterials

    Cite this

    Fibronectin-calcium phosphate composite layer on hydroxyapatite to enhance adhesion, cell spread and osteogenic differentiation of human mesenchymal stem cells in vitro. / Sogo, Yu; Ito, Atsuo; Matsuno, Tomonori; Oyane, Ayako; Tamazawa, Gaku; Satoh, Tazuko; Yamazaki, Atsushi; Uchimura, Eiji; Ohno, Tadao.

    In: Biomedical Materials, Vol. 2, No. 2, 009, 01.06.2007, p. 116-123.

    Research output: Contribution to journalArticle

    Sogo, Yu ; Ito, Atsuo ; Matsuno, Tomonori ; Oyane, Ayako ; Tamazawa, Gaku ; Satoh, Tazuko ; Yamazaki, Atsushi ; Uchimura, Eiji ; Ohno, Tadao. / Fibronectin-calcium phosphate composite layer on hydroxyapatite to enhance adhesion, cell spread and osteogenic differentiation of human mesenchymal stem cells in vitro. In: Biomedical Materials. 2007 ; Vol. 2, No. 2. pp. 116-123.
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    abstract = "Fibronectin (Fn) and type I collagen (Col) were immobilized on a surface of a hydroxyapatite (HAP) ceramic by coprecipitation with calcium phosphate in a supersaturated calcium phosphate solution prepared by mixing clinically approved infusion fluids. These proteins and the calcium phosphate precipitate formed a composite surface layer. As a result, the proteins were immobilized firmly as not to be released completely for 3 d in a physiological salt solution. When human mesenchymal stem cells (hMSCs) were cultured on a HAP ceramic in a differentiation medium supplemented with dexamethasone, β-glycerophosphate and ascorbic acid, hMSCs spread well within 1 h. The alkaline phosphatase (ALP) activity of hMSCs cultured on the Fn-calcium phosphate composite layer significantly increased compared with that of hMSCs cultured on the untreated HAP ceramic. On the other hand, Col did not increase the ALP activity of hMSCs and no synergy between Fn and Col was observed. Therefore, the Fn-calcium phosphate composite layer formed on the HAP is useful for the enhancement of the spreading and osteogenic differentiation of hMSCs in vitro.",
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    AU - Satoh, Tazuko

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