Fifty-second analysis of oxidative stress markers in human plasma using amide-modified liquid chromatography chip

M. Isokawa, K. Nakanishi, D. H. Yoon, T. Sekiguchi, T. Funatsu, S. Shoji, M. Tsunoda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports 50-s liquid chromatography (LC) separation of oxidative stress markers using an amide-modified pillar array column (PAC). The amide modification enables a sufficient retention of highly polar compounds under hydrophilic interaction LC (HILIC) conditions, and five fluorescence-labeled biothiols, i.e., cysteine, homocysteine, glutathione, cysteinylglycine, and N-acetylcysteine, in human plasma could be rapidly separated. Since these biothiols are associated with many diseases, this newly developed method could be a useful tool for the diagnosis or clinical investigation of biothiol-related diseases.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages583-584
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16/10/916/10/13

Keywords

  • Fluorescence
  • Hydrophilic interaction liquid chromatography
  • Pillar array
  • Thiol

ASJC Scopus subject areas

  • Control and Systems Engineering

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