Fifty-second analysis of oxidative stress markers in human plasma using amide-modified liquid chromatography chip

M. Isokawa; K. Nakanishi; Donghyun Yoon; Tetsushi Sekiguchi; T. Funatsu; Shuichi Shoji; M. Tsunoda

Fifty-second analysis of oxidative stress markers in human plasma using amide-modified liquid chromatography chip

M. Isokawa, K. Nakanishi, Donghyun Yoon, Tetsushi Sekiguchi, T. Funatsu, Shuichi Shoji, M. Tsunoda

Research Organization for Nano & Life Innovation

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This paper reports 50-s liquid chromatography (LC) separation of oxidative stress markers using an amide-modified pillar array column (PAC). The amide modification enables a sufficient retention of highly polar compounds under hydrophilic interaction LC (HILIC) conditions, and five fluorescence-labeled biothiols, i.e., cysteine, homocysteine, glutathione, cysteinylglycine, and N-acetylcysteine, in human plasma could be rapidly separated. Since these biothiols are associated with many diseases, this newly developed method could be a useful tool for the diagnosis or clinical investigation of biothiol-related diseases.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society
Pages	583-584
Number of pages	2
ISBN (Electronic)	9780979806490
Publication status	Published - 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 2016 Oct 9 → 2016 Oct 13

Other

Other	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country	Ireland
City	Dublin
Period	16/10/9 → 16/10/13

Keywords

Fluorescence
Hydrophilic interaction liquid chromatography
Pillar array
Thiol

ASJC Scopus subject areas

Control and Systems Engineering

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Isokawa, M., Nakanishi, K., Yoon, D., Sekiguchi, T., Funatsu, T., Shoji, S., & Tsunoda, M. (2016). Fifty-second analysis of oxidative stress markers in human plasma using amide-modified liquid chromatography chip. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 583-584). Chemical and Biological Microsystems Society.

Fifty-second analysis of oxidative stress markers in human plasma using amide-modified liquid chromatography chip. / Isokawa, M.; Nakanishi, K.; Yoon, Donghyun; Sekiguchi, Tetsushi; Funatsu, T.; Shoji, Shuichi; Tsunoda, M.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 583-584.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Isokawa, M, Nakanishi, K, Yoon, D, Sekiguchi, T, Funatsu, T, Shoji, S & Tsunoda, M 2016, Fifty-second analysis of oxidative stress markers in human plasma using amide-modified liquid chromatography chip. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, pp. 583-584, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 16/10/9.

Isokawa, M. ; Nakanishi, K. ; Yoon, Donghyun ; Sekiguchi, Tetsushi ; Funatsu, T. ; Shoji, Shuichi ; Tsunoda, M. / Fifty-second analysis of oxidative stress markers in human plasma using amide-modified liquid chromatography chip. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 583-584

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AB - This paper reports 50-s liquid chromatography (LC) separation of oxidative stress markers using an amide-modified pillar array column (PAC). The amide modification enables a sufficient retention of highly polar compounds under hydrophilic interaction LC (HILIC) conditions, and five fluorescence-labeled biothiols, i.e., cysteine, homocysteine, glutathione, cysteinylglycine, and N-acetylcysteine, in human plasma could be rapidly separated. Since these biothiols are associated with many diseases, this newly developed method could be a useful tool for the diagnosis or clinical investigation of biothiol-related diseases.

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