Fifty-second analysis of oxidative stress markers in human plasma using amide-modified liquid chromatography chip

M. Isokawa, K. Nakanishi, Donghyun Yoon, Tetsushi Sekiguchi, T. Funatsu, Shuichi Shoji, M. Tsunoda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports 50-s liquid chromatography (LC) separation of oxidative stress markers using an amide-modified pillar array column (PAC). The amide modification enables a sufficient retention of highly polar compounds under hydrophilic interaction LC (HILIC) conditions, and five fluorescence-labeled biothiols, i.e., cysteine, homocysteine, glutathione, cysteinylglycine, and N-acetylcysteine, in human plasma could be rapidly separated. Since these biothiols are associated with many diseases, this newly developed method could be a useful tool for the diagnosis or clinical investigation of biothiol-related diseases.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages583-584
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16/10/916/10/13

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Keywords

  • Fluorescence
  • Hydrophilic interaction liquid chromatography
  • Pillar array
  • Thiol

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Isokawa, M., Nakanishi, K., Yoon, D., Sekiguchi, T., Funatsu, T., Shoji, S., & Tsunoda, M. (2016). Fifty-second analysis of oxidative stress markers in human plasma using amide-modified liquid chromatography chip. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 583-584). Chemical and Biological Microsystems Society.