Abstract
This paper reports 50-s liquid chromatography (LC) separation of oxidative stress markers using an amide-modified pillar array column (PAC). The amide modification enables a sufficient retention of highly polar compounds under hydrophilic interaction LC (HILIC) conditions, and five fluorescence-labeled biothiols, i.e., cysteine, homocysteine, glutathione, cysteinylglycine, and N-acetylcysteine, in human plasma could be rapidly separated. Since these biothiols are associated with many diseases, this newly developed method could be a useful tool for the diagnosis or clinical investigation of biothiol-related diseases.
| Original language | English |
|---|---|
| Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 583-584 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780979806490 |
| Publication status | Published - 2016 |
| Event | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 2016 Oct 9 → 2016 Oct 13 |
Other
| Other | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
|---|---|
| Country | Ireland |
| City | Dublin |
| Period | 16/10/9 → 16/10/13 |
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Keywords
- Fluorescence
- Hydrophilic interaction liquid chromatography
- Pillar array
- Thiol
ASJC Scopus subject areas
- Control and Systems Engineering
Cite this
Isokawa, M., Nakanishi, K., Yoon, D., Sekiguchi, T., Funatsu, T., Shoji, S., & Tsunoda, M. (2016). Fifty-second analysis of oxidative stress markers in human plasma using amide-modified liquid chromatography chip. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 583-584). Chemical and Biological Microsystems Society.