TY - GEN
T1 - Fifty-second analysis of oxidative stress markers in human plasma using amide-modified liquid chromatography chip
AU - Isokawa, M.
AU - Nakanishi, K.
AU - Yoon, D. H.
AU - Sekiguchi, T.
AU - Funatsu, T.
AU - Shoji, S.
AU - Tsunoda, M.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016
Y1 - 2016
N2 - This paper reports 50-s liquid chromatography (LC) separation of oxidative stress markers using an amide-modified pillar array column (PAC). The amide modification enables a sufficient retention of highly polar compounds under hydrophilic interaction LC (HILIC) conditions, and five fluorescence-labeled biothiols, i.e., cysteine, homocysteine, glutathione, cysteinylglycine, and N-acetylcysteine, in human plasma could be rapidly separated. Since these biothiols are associated with many diseases, this newly developed method could be a useful tool for the diagnosis or clinical investigation of biothiol-related diseases.
AB - This paper reports 50-s liquid chromatography (LC) separation of oxidative stress markers using an amide-modified pillar array column (PAC). The amide modification enables a sufficient retention of highly polar compounds under hydrophilic interaction LC (HILIC) conditions, and five fluorescence-labeled biothiols, i.e., cysteine, homocysteine, glutathione, cysteinylglycine, and N-acetylcysteine, in human plasma could be rapidly separated. Since these biothiols are associated with many diseases, this newly developed method could be a useful tool for the diagnosis or clinical investigation of biothiol-related diseases.
KW - Fluorescence
KW - Hydrophilic interaction liquid chromatography
KW - Pillar array
KW - Thiol
UR - http://www.scopus.com/inward/record.url?scp=85014163863&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014163863&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85014163863
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 583
EP - 584
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Y2 - 9 October 2016 through 13 October 2016
ER -