Filament formation and robust strand exchange activities of the rice DMC1A and DMC1B proteins

Isao Sakane, Chiaki Kamataki, Yoshimasa Takizawa, Marina Nakashima, Seiichi Toki, Hiroaki Ichikawa, Shukuko Ikawa, Takehiko Shibata, Hitoshi Kurumizaka

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    The DMC1 protein, a meiosis-specific DNA recombinase, catalyzes strand exchange between homologous chromosomes. In rice, two Dmc1 genes, Dmc1A and Dmc1B, have been reported. Although the Oryza sativa DMC1A protein has been partially characterized, however the biochemical properties of the DMC1B protein have not been defined. In the present study, we expressed the Oryza sativa DMC1A and DMC1B proteins in bacteria and purified them. The purified DMC1A and DMC1B proteins formed helical filaments along single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA), and promoted robust strand exchange between ssDNA and dsDNA over five thousand base pairs in the presence of RPA, as a co-factor. The DMC1A and DMC1B proteins also promoted strand exchange in the absence of RPA with long DNA substrates containing several thousand base pairs. In contrast, the human DMC1 protein strictly required RPA to promote strand exchange with these long DNA substrates. The strand-exchange activity of the Oryza sativa DMC1A protein was much higher than that of the DMC1B protein. Consistently, the DNA-binding activity of the DMC1A protein was higher than that of the DMC1B protein. These biochemical differences between the DMC1A and DMC1B proteins may provide important insight into their functional differences during meiosis in rice.

    Original languageEnglish
    Pages (from-to)4266-4276
    Number of pages11
    JournalNucleic Acids Research
    Volume36
    Issue number13
    DOIs
    Publication statusPublished - 2008 Aug

    Fingerprint

    Proteins
    Meiosis
    Base Pairing
    Oryza
    Recombinases
    Single-Stranded DNA
    Chromosomes
    Bacteria
    DNA
    Genes

    ASJC Scopus subject areas

    • Genetics

    Cite this

    Sakane, I., Kamataki, C., Takizawa, Y., Nakashima, M., Toki, S., Ichikawa, H., ... Kurumizaka, H. (2008). Filament formation and robust strand exchange activities of the rice DMC1A and DMC1B proteins. Nucleic Acids Research, 36(13), 4266-4276. https://doi.org/10.1093/nar/gkn405

    Filament formation and robust strand exchange activities of the rice DMC1A and DMC1B proteins. / Sakane, Isao; Kamataki, Chiaki; Takizawa, Yoshimasa; Nakashima, Marina; Toki, Seiichi; Ichikawa, Hiroaki; Ikawa, Shukuko; Shibata, Takehiko; Kurumizaka, Hitoshi.

    In: Nucleic Acids Research, Vol. 36, No. 13, 08.2008, p. 4266-4276.

    Research output: Contribution to journalArticle

    Sakane, I, Kamataki, C, Takizawa, Y, Nakashima, M, Toki, S, Ichikawa, H, Ikawa, S, Shibata, T & Kurumizaka, H 2008, 'Filament formation and robust strand exchange activities of the rice DMC1A and DMC1B proteins', Nucleic Acids Research, vol. 36, no. 13, pp. 4266-4276. https://doi.org/10.1093/nar/gkn405
    Sakane I, Kamataki C, Takizawa Y, Nakashima M, Toki S, Ichikawa H et al. Filament formation and robust strand exchange activities of the rice DMC1A and DMC1B proteins. Nucleic Acids Research. 2008 Aug;36(13):4266-4276. https://doi.org/10.1093/nar/gkn405
    Sakane, Isao ; Kamataki, Chiaki ; Takizawa, Yoshimasa ; Nakashima, Marina ; Toki, Seiichi ; Ichikawa, Hiroaki ; Ikawa, Shukuko ; Shibata, Takehiko ; Kurumizaka, Hitoshi. / Filament formation and robust strand exchange activities of the rice DMC1A and DMC1B proteins. In: Nucleic Acids Research. 2008 ; Vol. 36, No. 13. pp. 4266-4276.
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