Fish sleeping under sandy bottom: Interplay of melatonin and clock genes

Sung Pyo Hur, Yuki Takeuchi, Hitomi Itoh, Miyuki Uchimura, Kai Takahashi, Hyeong Cheol Kang, Young Don Lee, Se Jae Kim, Akihiro Takemura

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Wrasse species exhibit a definite daily rhythm in locomotor activity and bury themselves in the sand at the bottom of the ocean at night. It remains unclear how their behavior in locomotor activity is endogenously regulated. The aim of the present study was to clarify the involvement of melatonin and clock genes (Per1, Per2, Bmal1, and Cry1) in daily and circadian rhythms of the threespot wrasse, Halichoeres trimaculatus, which is a common species in coral reefs. Daily and circadian rhythms in locomotor activity were monitored under conditions of light-dark cycle (LD = 12:12), constant light (LL), and darkness (DD). Daily rhythms in locomotor activity were observed under LD and persisted under LL and DD. Melatonin from a cultured pineal gland showed daily variations with an increase during the nighttime and a decrease during daytime, which persisted under DD. Melatonin treatment induced decreases in locomotor activity and respiratory rate, suggesting that melatonin has a sleep-inducing effect. Per1 and Per2 mRNA abundance in the brain under LD showed daily rhythms with an increase around lights on. Robust oscillation of Per1 and Per2 mRNA expression persisted under DD and LL, respectively. Expression of Bmal1 and Cry1 mRNA also showed daily and circadian patterns. These results suggest that clock genes are related to circadian rhythms in locomotor activity and that melatonin plays a role in inducing a sleep-like state after fish bury themselves in the sand. We conclude that the sleep-wake rhythm of the wrasse is regulated by a coordination of melatonin and clock genes.

Original languageEnglish
Pages (from-to)37-45
Number of pages9
JournalGeneral and Comparative Endocrinology
Volume177
Issue number1
DOIs
Publication statusPublished - 2012 May 15
Externally publishedYes

Fingerprint

melatonin
Melatonin
Locomotion
locomotion
Fishes
Labridae
Circadian Rhythm
sleep
fish
circadian rhythm
Genes
Sleep
genes
Messenger RNA
sand
Coral Reefs
Light
pineal body
Pineal Gland
Darkness

Keywords

  • Circadian
  • Clock genes
  • Locomotor activity
  • Melatonin
  • Rhythm
  • Sleep
  • Wrasse

ASJC Scopus subject areas

  • Endocrinology

Cite this

Hur, S. P., Takeuchi, Y., Itoh, H., Uchimura, M., Takahashi, K., Kang, H. C., ... Takemura, A. (2012). Fish sleeping under sandy bottom: Interplay of melatonin and clock genes. General and Comparative Endocrinology, 177(1), 37-45. https://doi.org/10.1016/j.ygcen.2012.01.007

Fish sleeping under sandy bottom : Interplay of melatonin and clock genes. / Hur, Sung Pyo; Takeuchi, Yuki; Itoh, Hitomi; Uchimura, Miyuki; Takahashi, Kai; Kang, Hyeong Cheol; Lee, Young Don; Kim, Se Jae; Takemura, Akihiro.

In: General and Comparative Endocrinology, Vol. 177, No. 1, 15.05.2012, p. 37-45.

Research output: Contribution to journalArticle

Hur, SP, Takeuchi, Y, Itoh, H, Uchimura, M, Takahashi, K, Kang, HC, Lee, YD, Kim, SJ & Takemura, A 2012, 'Fish sleeping under sandy bottom: Interplay of melatonin and clock genes', General and Comparative Endocrinology, vol. 177, no. 1, pp. 37-45. https://doi.org/10.1016/j.ygcen.2012.01.007
Hur, Sung Pyo ; Takeuchi, Yuki ; Itoh, Hitomi ; Uchimura, Miyuki ; Takahashi, Kai ; Kang, Hyeong Cheol ; Lee, Young Don ; Kim, Se Jae ; Takemura, Akihiro. / Fish sleeping under sandy bottom : Interplay of melatonin and clock genes. In: General and Comparative Endocrinology. 2012 ; Vol. 177, No. 1. pp. 37-45.
@article{895d1ea046094ded8c1831192311d1f6,
title = "Fish sleeping under sandy bottom: Interplay of melatonin and clock genes",
abstract = "Wrasse species exhibit a definite daily rhythm in locomotor activity and bury themselves in the sand at the bottom of the ocean at night. It remains unclear how their behavior in locomotor activity is endogenously regulated. The aim of the present study was to clarify the involvement of melatonin and clock genes (Per1, Per2, Bmal1, and Cry1) in daily and circadian rhythms of the threespot wrasse, Halichoeres trimaculatus, which is a common species in coral reefs. Daily and circadian rhythms in locomotor activity were monitored under conditions of light-dark cycle (LD = 12:12), constant light (LL), and darkness (DD). Daily rhythms in locomotor activity were observed under LD and persisted under LL and DD. Melatonin from a cultured pineal gland showed daily variations with an increase during the nighttime and a decrease during daytime, which persisted under DD. Melatonin treatment induced decreases in locomotor activity and respiratory rate, suggesting that melatonin has a sleep-inducing effect. Per1 and Per2 mRNA abundance in the brain under LD showed daily rhythms with an increase around lights on. Robust oscillation of Per1 and Per2 mRNA expression persisted under DD and LL, respectively. Expression of Bmal1 and Cry1 mRNA also showed daily and circadian patterns. These results suggest that clock genes are related to circadian rhythms in locomotor activity and that melatonin plays a role in inducing a sleep-like state after fish bury themselves in the sand. We conclude that the sleep-wake rhythm of the wrasse is regulated by a coordination of melatonin and clock genes.",
keywords = "Circadian, Clock genes, Locomotor activity, Melatonin, Rhythm, Sleep, Wrasse",
author = "Hur, {Sung Pyo} and Yuki Takeuchi and Hitomi Itoh and Miyuki Uchimura and Kai Takahashi and Kang, {Hyeong Cheol} and Lee, {Young Don} and Kim, {Se Jae} and Akihiro Takemura",
year = "2012",
month = "5",
day = "15",
doi = "10.1016/j.ygcen.2012.01.007",
language = "English",
volume = "177",
pages = "37--45",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Fish sleeping under sandy bottom

T2 - Interplay of melatonin and clock genes

AU - Hur, Sung Pyo

AU - Takeuchi, Yuki

AU - Itoh, Hitomi

AU - Uchimura, Miyuki

AU - Takahashi, Kai

AU - Kang, Hyeong Cheol

AU - Lee, Young Don

AU - Kim, Se Jae

AU - Takemura, Akihiro

PY - 2012/5/15

Y1 - 2012/5/15

N2 - Wrasse species exhibit a definite daily rhythm in locomotor activity and bury themselves in the sand at the bottom of the ocean at night. It remains unclear how their behavior in locomotor activity is endogenously regulated. The aim of the present study was to clarify the involvement of melatonin and clock genes (Per1, Per2, Bmal1, and Cry1) in daily and circadian rhythms of the threespot wrasse, Halichoeres trimaculatus, which is a common species in coral reefs. Daily and circadian rhythms in locomotor activity were monitored under conditions of light-dark cycle (LD = 12:12), constant light (LL), and darkness (DD). Daily rhythms in locomotor activity were observed under LD and persisted under LL and DD. Melatonin from a cultured pineal gland showed daily variations with an increase during the nighttime and a decrease during daytime, which persisted under DD. Melatonin treatment induced decreases in locomotor activity and respiratory rate, suggesting that melatonin has a sleep-inducing effect. Per1 and Per2 mRNA abundance in the brain under LD showed daily rhythms with an increase around lights on. Robust oscillation of Per1 and Per2 mRNA expression persisted under DD and LL, respectively. Expression of Bmal1 and Cry1 mRNA also showed daily and circadian patterns. These results suggest that clock genes are related to circadian rhythms in locomotor activity and that melatonin plays a role in inducing a sleep-like state after fish bury themselves in the sand. We conclude that the sleep-wake rhythm of the wrasse is regulated by a coordination of melatonin and clock genes.

AB - Wrasse species exhibit a definite daily rhythm in locomotor activity and bury themselves in the sand at the bottom of the ocean at night. It remains unclear how their behavior in locomotor activity is endogenously regulated. The aim of the present study was to clarify the involvement of melatonin and clock genes (Per1, Per2, Bmal1, and Cry1) in daily and circadian rhythms of the threespot wrasse, Halichoeres trimaculatus, which is a common species in coral reefs. Daily and circadian rhythms in locomotor activity were monitored under conditions of light-dark cycle (LD = 12:12), constant light (LL), and darkness (DD). Daily rhythms in locomotor activity were observed under LD and persisted under LL and DD. Melatonin from a cultured pineal gland showed daily variations with an increase during the nighttime and a decrease during daytime, which persisted under DD. Melatonin treatment induced decreases in locomotor activity and respiratory rate, suggesting that melatonin has a sleep-inducing effect. Per1 and Per2 mRNA abundance in the brain under LD showed daily rhythms with an increase around lights on. Robust oscillation of Per1 and Per2 mRNA expression persisted under DD and LL, respectively. Expression of Bmal1 and Cry1 mRNA also showed daily and circadian patterns. These results suggest that clock genes are related to circadian rhythms in locomotor activity and that melatonin plays a role in inducing a sleep-like state after fish bury themselves in the sand. We conclude that the sleep-wake rhythm of the wrasse is regulated by a coordination of melatonin and clock genes.

KW - Circadian

KW - Clock genes

KW - Locomotor activity

KW - Melatonin

KW - Rhythm

KW - Sleep

KW - Wrasse

UR - http://www.scopus.com/inward/record.url?scp=84860537442&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860537442&partnerID=8YFLogxK

U2 - 10.1016/j.ygcen.2012.01.007

DO - 10.1016/j.ygcen.2012.01.007

M3 - Article

C2 - 22285600

AN - SCOPUS:84860537442

VL - 177

SP - 37

EP - 45

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

IS - 1

ER -