Flow immunoassay for detection of human chorionic gonadotrophin using a cation exchange resin packed capillary column

Noriyuki Nakamura, Tae Kyu Lim, Jae Mun Jeong, Tadashi Matsunaga

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24 Citations (Scopus)

Abstract

A human chorionic gonadotrophin (HCG) detection was carried out by the flow immunoassay system with a cation exchange resin packed capillary column (762 μm i.d. × 10 cm length), that can be considered a miniaturised version of the previously reported system. The HCG and fluorescein isothiocyanate (FITC) conjugated immunoglobulin G (IgG) antibody complex was separated from free FITC conjugated IgG on the basis of differences in isoelectric points. The fluorescence intensity correlated linearly with concentrations of HCG in the range of 25-1500 mIU/ml. This simple technique permits the assay of HCG within 1.3 min. It was possible to reuse of free FITC conjugated IgG. This simple and rapid immunoassay method can be used for the continuous detection of HCG.

Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalAnalytica Chimica Acta
Volume439
Issue number1
DOIs
Publication statusPublished - 2001 Jul 17
Externally publishedYes

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Keywords

  • Capillary column
  • Continuous detection
  • Flow immunoassay
  • Fluorescein isothiocyanate conjugated immunoglobulin G
  • Human chorionic gonadotrophin

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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