Abstract
A simple and rapid detection system for wheat allergen was developed based on a luminescence immunoassay with a continuous flow system. Wheat allergen and alkaline phosphatase conjugated IgE (ALP-IgE) was separated from free ALP-IgE on the basis of a difference in isoelectric point, using with an anion exchange resin. Luminescence output of the assay correlated linearly with the concentration of allergen in the range of 1-100 μg/ml. Reuse of free ALP-IgE was possible.
| Original language | English |
|---|---|
| Pages (from-to) | 445-449 |
| Number of pages | 5 |
| Journal | Biotechnology Techniques |
| Volume | 12 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Biochemistry
Cite this
Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE. / Lim, Tae Kyu; Nakamura, Noriyuki; Matsunaga, Tadashi.
In: Biotechnology Techniques, Vol. 12, No. 6, 1998, p. 445-449.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE
AU - Lim, Tae Kyu
AU - Nakamura, Noriyuki
AU - Matsunaga, Tadashi
PY - 1998
Y1 - 1998
N2 - A simple and rapid detection system for wheat allergen was developed based on a luminescence immunoassay with a continuous flow system. Wheat allergen and alkaline phosphatase conjugated IgE (ALP-IgE) was separated from free ALP-IgE on the basis of a difference in isoelectric point, using with an anion exchange resin. Luminescence output of the assay correlated linearly with the concentration of allergen in the range of 1-100 μg/ml. Reuse of free ALP-IgE was possible.
AB - A simple and rapid detection system for wheat allergen was developed based on a luminescence immunoassay with a continuous flow system. Wheat allergen and alkaline phosphatase conjugated IgE (ALP-IgE) was separated from free ALP-IgE on the basis of a difference in isoelectric point, using with an anion exchange resin. Luminescence output of the assay correlated linearly with the concentration of allergen in the range of 1-100 μg/ml. Reuse of free ALP-IgE was possible.
UR - http://www.scopus.com/inward/record.url?scp=0031903679&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031903679&partnerID=8YFLogxK
U2 - 10.1023/A:1008803221243
DO - 10.1023/A:1008803221243
M3 - Article
AN - SCOPUS:0031903679
VL - 12
SP - 445
EP - 449
JO - Biotechnology Letters
JF - Biotechnology Letters
SN - 0141-5492
IS - 6
ER -