Abstract
A simple and rapid detection system for wheat allergen was developed based on a luminescence immunoassay with a continuous flow system. Wheat allergen and alkaline phosphatase conjugated IgE (ALP-IgE) was separated from free ALP-IgE on the basis of a difference in isoelectric point, using with an anion exchange resin. Luminescence output of the assay correlated linearly with the concentration of allergen in the range of 1-100 μg/ml. Reuse of free ALP-IgE was possible.
| Original language | English |
|---|---|
| Pages (from-to) | 445-449 |
| Number of pages | 5 |
| Journal | Biotechnology Techniques |
| Volume | 12 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1998 |
| Externally published | Yes |
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Biochemistry
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Cite this
Lim, T. K., Nakamura, N., & Matsunaga, T. (1998). Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE. Biotechnology Techniques, 12(6), 445-449. https://doi.org/10.1023/A:1008803221243