Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE

Tae Kyu Lim, Noriyuki Nakamura, Tadashi Matsunaga

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A simple and rapid detection system for wheat allergen was developed based on a luminescence immunoassay with a continuous flow system. Wheat allergen and alkaline phosphatase conjugated IgE (ALP-IgE) was separated from free ALP-IgE on the basis of a difference in isoelectric point, using with an anion exchange resin. Luminescence output of the assay correlated linearly with the concentration of allergen in the range of 1-100 μg/ml. Reuse of free ALP-IgE was possible.

Original languageEnglish
Pages (from-to)445-449
Number of pages5
JournalBiotechnology Techniques
Volume12
Issue number6
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Allergens
Phosphatases
Immunoassay
Immunoglobulin E
Triticum
Anions
Alkaline Phosphatase
Ion exchange
Negative ions
Luminescence
Anion Exchange Resins
Isoelectric Point
Assays
Resins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

Cite this

Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE. / Lim, Tae Kyu; Nakamura, Noriyuki; Matsunaga, Tadashi.

In: Biotechnology Techniques, Vol. 12, No. 6, 1998, p. 445-449.

Research output: Contribution to journalArticle

Lim, Tae Kyu ; Nakamura, Noriyuki ; Matsunaga, Tadashi. / Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE. In: Biotechnology Techniques. 1998 ; Vol. 12, No. 6. pp. 445-449.
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