Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE

Tae Kyu Lim; Noriyuki Nakamura; Tadashi Matsunaga

doi:10.1023/A:1008803221243

Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE

Tae Kyu Lim, Noriyuki Nakamura, Tadashi Matsunaga

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A simple and rapid detection system for wheat allergen was developed based on a luminescence immunoassay with a continuous flow system. Wheat allergen and alkaline phosphatase conjugated IgE (ALP-IgE) was separated from free ALP-IgE on the basis of a difference in isoelectric point, using with an anion exchange resin. Luminescence output of the assay correlated linearly with the concentration of allergen in the range of 1-100 μg/ml. Reuse of free ALP-IgE was possible.

Original language	English
Pages (from-to)	445-449
Number of pages	5
Journal	Biotechnology Techniques
Volume	12
Issue number	6
DOIs	https://doi.org/10.1023/A:1008803221243
Publication status	Published - 1998
Externally published	Yes

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Biochemistry

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AU - Lim, Tae Kyu

AU - Nakamura, Noriyuki

AU - Matsunaga, Tadashi

PY - 1998

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AB - A simple and rapid detection system for wheat allergen was developed based on a luminescence immunoassay with a continuous flow system. Wheat allergen and alkaline phosphatase conjugated IgE (ALP-IgE) was separated from free ALP-IgE on the basis of a difference in isoelectric point, using with an anion exchange resin. Luminescence output of the assay correlated linearly with the concentration of allergen in the range of 1-100 μg/ml. Reuse of free ALP-IgE was possible.

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ER -

Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE

Abstract

ASJC Scopus subject areas

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