Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE

Tae Kyu Lim, Noriyuki Nakamura, Tadashi Matsunaga

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2 Citations (Scopus)

Abstract

A simple and rapid detection system for wheat allergen was developed based on a luminescence immunoassay with a continuous flow system. Wheat allergen and alkaline phosphatase conjugated IgE (ALP-IgE) was separated from free ALP-IgE on the basis of a difference in isoelectric point, using with an anion exchange resin. Luminescence output of the assay correlated linearly with the concentration of allergen in the range of 1-100 μg/ml. Reuse of free ALP-IgE was possible.

Original languageEnglish
Pages (from-to)445-449
Number of pages5
JournalBiotechnology Techniques
Volume12
Issue number6
DOIs
Publication statusPublished - 1998
Externally publishedYes

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ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

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