Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE

Tae Kyu Lim; Noriyuki Nakamura; Tadashi Matsunaga

doi:10.1023/A:1008803221243

Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE

Tae Kyu Lim^*, Noriyuki Nakamura, Tadashi Matsunaga

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A simple and rapid detection system for wheat allergen was developed based on a luminescence immunoassay with a continuous flow system. Wheat allergen and alkaline phosphatase conjugated IgE (ALP-IgE) was separated from free ALP-IgE on the basis of a difference in isoelectric point, using with an anion exchange resin. Luminescence output of the assay correlated linearly with the concentration of allergen in the range of 1-100 μg/ml. Reuse of free ALP-IgE was possible.

Original language	English
Pages (from-to)	445-449
Number of pages	5
Journal	Biotechnology Techniques
Volume	12
Issue number	6
DOIs	https://doi.org/10.1023/A:1008803221243
Publication status	Published - 1998
Externally published	Yes

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Biochemistry

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title = "Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE",

abstract = "A simple and rapid detection system for wheat allergen was developed based on a luminescence immunoassay with a continuous flow system. Wheat allergen and alkaline phosphatase conjugated IgE (ALP-IgE) was separated from free ALP-IgE on the basis of a difference in isoelectric point, using with an anion exchange resin. Luminescence output of the assay correlated linearly with the concentration of allergen in the range of 1-100 μg/ml. Reuse of free ALP-IgE was possible.",

author = "Lim, {Tae Kyu} and Noriyuki Nakamura and Tadashi Matsunaga",

year = "1998",

doi = "10.1023/A:1008803221243",

language = "English",

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pages = "445--449",

journal = "Biotechnology Letters",

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T1 - Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE

AU - Lim, Tae Kyu

AU - Nakamura, Noriyuki

AU - Matsunaga, Tadashi

PY - 1998

Y1 - 1998

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AB - A simple and rapid detection system for wheat allergen was developed based on a luminescence immunoassay with a continuous flow system. Wheat allergen and alkaline phosphatase conjugated IgE (ALP-IgE) was separated from free ALP-IgE on the basis of a difference in isoelectric point, using with an anion exchange resin. Luminescence output of the assay correlated linearly with the concentration of allergen in the range of 1-100 μg/ml. Reuse of free ALP-IgE was possible.

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JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

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ER -

Flow immunoassay for detection of wheat allergen using an anion exchange column and alkaline phosphatase conjugated IgE

Abstract

ASJC Scopus subject areas

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