Abstract
A simple and rapid detection system for wheat allergen was developed based on a luminescence immunoassay with a continuous flow system. Wheat allergen and alkaline phosphatase conjugated IgE (ALP-IgE) was separated from free ALP-IgE on the basis of a difference in isoelectric point, using with an anion exchange resin. Luminescence output of the assay correlated linearly with the concentration of allergen in the range of 1-100 μg/ml. Reuse of free ALP-IgE was possible.
Original language | English |
---|---|
Pages (from-to) | 445-449 |
Number of pages | 5 |
Journal | Biotechnology Techniques |
Volume | 12 |
Issue number | 6 |
DOIs | |
Publication status | Published - 1998 |
Externally published | Yes |
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Biochemistry