Flow immunoassay using a cation exchange column and fluorescence-labelled antibody for detection of allergen

Tae Kyu Lim, Noriyuki Nakamura, Tadashi Matsunaga

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An allergen detection was carried out based on a fluorescence immunoassay with a flow injection system. Allergen and fluorescein isothiocyanate (FITC) conjugated IgE antibody complex was separated from free FITC conjugated IgE antibody using a cation exchange column. The fluorescence intensity correlated linearly with a concentration of allergen in the range of 0.01-2.0 mg ml-1. Reuse of free FITC conjugated IgE was possible. This simple and rapid immunoassay method can be used for the continuous detection of allergen.

Original languageEnglish
Pages (from-to)29-34
Number of pages6
JournalAnalytica Chimica Acta
Issue number1-3
Publication statusPublished - 1997 Nov 10
Externally publishedYes



  • Allergen detection
  • Antibody
  • Cation exchange column
  • Flow injection
  • Fluorescence
  • Immunoassay

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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