Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes

Kazuhiro Furukawa; Hiroshi Abe; Yasutsugu Tamura; Rei Yoshimoto; Minoru Yoshida; Satoshi Tsuneda; Yoshihiro Ito

doi:10.1002/anie.201104425

Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes

Kazuhiro Furukawa, Hiroshi Abe^*, Yasutsugu Tamura, Rei Yoshimoto, Minoru Yoshida, Satoshi Tsuneda, Yoshihiro Ito

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Fluorescence through proximity: Reduction-triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence-specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method.

Original language	English
Pages (from-to)	12020-12023
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	50
DOIs	https://doi.org/10.1002/anie.201104425
Publication status	Published - 2011 Dec 9

Keywords

RNA structures
biosensors
fluorescent probes
lariat RNA
oligonucleotides

ASJC Scopus subject areas

Catalysis
Chemistry(all)

Access to Document

10.1002/anie.201104425

Cite this

@article{fd952f3e07484d48965e953c18b4d4b5,

title = "Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes",

abstract = "Fluorescence through proximity: Reduction-triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence-specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method.",

keywords = "RNA structures, biosensors, fluorescent probes, lariat RNA, oligonucleotides",

author = "Kazuhiro Furukawa and Hiroshi Abe and Yasutsugu Tamura and Rei Yoshimoto and Minoru Yoshida and Satoshi Tsuneda and Yoshihiro Ito",

year = "2011",

month = dec,

day = "9",

doi = "10.1002/anie.201104425",

language = "English",

volume = "50",

pages = "12020--12023",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley and Sons Ltd",

number = "50",

}

TY - JOUR

T1 - Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes

AU - Furukawa, Kazuhiro

AU - Abe, Hiroshi

AU - Tamura, Yasutsugu

AU - Yoshimoto, Rei

AU - Yoshida, Minoru

AU - Tsuneda, Satoshi

AU - Ito, Yoshihiro

PY - 2011/12/9

Y1 - 2011/12/9

N2 - Fluorescence through proximity: Reduction-triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence-specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method.

AB - Fluorescence through proximity: Reduction-triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence-specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method.

KW - RNA structures

KW - biosensors

KW - fluorescent probes

KW - lariat RNA

KW - oligonucleotides

UR - http://www.scopus.com/inward/record.url?scp=83455197064&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=83455197064&partnerID=8YFLogxK

U2 - 10.1002/anie.201104425

DO - 10.1002/anie.201104425

M3 - Article

C2 - 22021091

AN - SCOPUS:83455197064

VL - 50

SP - 12020

EP - 12023

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

SN - 1433-7851

IS - 50

ER -

Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this