Abstract
Fluorescence through proximity: Reduction-triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence-specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method.
| Original language | English |
|---|---|
| Pages (from-to) | 12020-12023 |
| Number of pages | 4 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 50 |
| Issue number | 50 |
| DOIs | |
| Publication status | Published - 2011 Dec 9 |
Keywords
- RNA structures
- biosensors
- fluorescent probes
- lariat RNA
- oligonucleotides
ASJC Scopus subject areas
- Catalysis
- Chemistry(all)
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Cite this
Furukawa, K., Abe, H., Tamura, Y., Yoshimoto, R., Yoshida, M., Tsuneda, S., & Ito, Y. (2011). Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes. Angewandte Chemie - International Edition, 50(50), 12020-12023. https://doi.org/10.1002/anie.201104425