Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes

Kazuhiro Furukawa, Hiroshi Abe, Yasutsugu Tamura, Rei Yoshimoto, Minoru Yoshida, Satoshi Tsuneda, Yoshihiro Ito

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Fluorescence through proximity: Reduction-triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence-specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method.

Original languageEnglish
Pages (from-to)12020-12023
Number of pages4
JournalAngewandte Chemie - International Edition
Volume50
Issue number50
DOIs
Publication statusPublished - 2011 Dec 9

Keywords

  • RNA structures
  • biosensors
  • fluorescent probes
  • lariat RNA
  • oligonucleotides

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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