Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes

Kazuhiro Furukawa; Hiroshi Abe; Yasutsugu Tamura; Rei Yoshimoto; Minoru Yoshida; Satoshi Tsuneda; Yoshihiro Ito

doi:10.1002/anie.201104425

Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes

Kazuhiro Furukawa, Hiroshi Abe^*, Yasutsugu Tamura, Rei Yoshimoto, Minoru Yoshida, Satoshi Tsuneda, Yoshihiro Ito

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Fluorescence through proximity: Reduction-triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence-specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method.

Original language	English
Pages (from-to)	12020-12023
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	50
DOIs	https://doi.org/10.1002/anie.201104425
Publication status	Published - 2011 Dec 9

Keywords

RNA structures
biosensors
fluorescent probes
lariat RNA
oligonucleotides

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.201104425

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Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes. / Furukawa, Kazuhiro; Abe, Hiroshi; Tamura, Yasutsugu; Yoshimoto, Rei; Yoshida, Minoru; Tsuneda, Satoshi; Ito, Yoshihiro.

In: Angewandte Chemie - International Edition, Vol. 50, No. 50, 09.12.2011, p. 12020-12023.

Research output: Contribution to journal › Article › peer-review

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Fluorescence detection of intron lariat RNA with reduction-triggered fluorescent probes

Abstract

Keywords

ASJC Scopus subject areas

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