Fluorescence generation from tandem repeats of a malachite green RNA aptamer using rolling circle transcription

Kazuhiro Furukawa, Hiroshi Abe, Naoko Abe, Mitsuru Harada, Satoshi Tsuneda, Yoshihiro Ito

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    We demonstrate a generation of tandem repeats of a malachite green (MG) RNA aptamer using rolling circle transcription. To keep the higher-order structure of each aptamer on long RNA, we designed a sequence of circular DNA with a 14-base linker. T7 RNA polymerase was superior to Escherichia coli RNA polymerase in the specific transcription of the MG RNA aptamer. Finally, the generation of the fluorescence signal was confirmed from aptamer repeats with MG.

    Original languageEnglish
    Pages (from-to)4562-4565
    Number of pages4
    JournalBioorganic and Medicinal Chemistry Letters
    Volume18
    Issue number16
    DOIs
    Publication statusPublished - 2008 Aug 15

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    Tandem Repeat Sequences
    Transcription
    Fluorescence
    Circular DNA
    DNA-Directed RNA Polymerases
    Escherichia coli
    RNA
    malachite green RNA aptamer
    malachite green
    bacteriophage T7 RNA polymerase

    Keywords

    • Amplification
    • Fluorescence
    • Nucleic acid
    • Polymerase
    • RNA

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Organic Chemistry
    • Drug Discovery
    • Pharmaceutical Science

    Cite this

    Fluorescence generation from tandem repeats of a malachite green RNA aptamer using rolling circle transcription. / Furukawa, Kazuhiro; Abe, Hiroshi; Abe, Naoko; Harada, Mitsuru; Tsuneda, Satoshi; Ito, Yoshihiro.

    In: Bioorganic and Medicinal Chemistry Letters, Vol. 18, No. 16, 15.08.2008, p. 4562-4565.

    Research output: Contribution to journalArticle

    Furukawa, Kazuhiro ; Abe, Hiroshi ; Abe, Naoko ; Harada, Mitsuru ; Tsuneda, Satoshi ; Ito, Yoshihiro. / Fluorescence generation from tandem repeats of a malachite green RNA aptamer using rolling circle transcription. In: Bioorganic and Medicinal Chemistry Letters. 2008 ; Vol. 18, No. 16. pp. 4562-4565.
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    AU - Tsuneda, Satoshi

    AU - Ito, Yoshihiro

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