Fluorescence generation from tandem repeats of a malachite green RNA aptamer using rolling circle transcription

Kazuhiro Furukawa, Hiroshi Abe, Naoko Abe, Mitsuru Harada, Satoshi Tsuneda, Yoshihiro Ito

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We demonstrate a generation of tandem repeats of a malachite green (MG) RNA aptamer using rolling circle transcription. To keep the higher-order structure of each aptamer on long RNA, we designed a sequence of circular DNA with a 14-base linker. T7 RNA polymerase was superior to Escherichia coli RNA polymerase in the specific transcription of the MG RNA aptamer. Finally, the generation of the fluorescence signal was confirmed from aptamer repeats with MG.

Original languageEnglish
Pages (from-to)4562-4565
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume18
Issue number16
DOIs
Publication statusPublished - 2008 Aug 15

Keywords

  • Amplification
  • Fluorescence
  • Nucleic acid
  • Polymerase
  • RNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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