Fluorescence imaging of Escherichia coli on a rotating optical disk

Takayuki Shima, Hideyuki Shiramizu, Makoto Fujimaki, Chiaki Kuroda, Yoshimichi Ohki

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    Abstract

    We constructed a fluorescence image from a rotating optical disk sample by aligning laser-scanned results based on positioning marks formed on the disk substrate. When Escherichia coli cells and 0.75-μm-diameter polystyrene beads were placed on the sample surface and a cell staining solution was used, fluorescence from only E. coli cells was observed in the obtained image. This indicates that E. coli cells can be distinguished from polystyrene beads, an abiotic substance, in one image. A promising utilization of this method is as a sensor for finding targets among contaminants on a large area.

    Original languageEnglish
    Article number088003
    JournalJapanese Journal of Applied Physics
    Volume57
    Issue number8
    DOIs
    Publication statusPublished - 2018 Aug 1

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    ASJC Scopus subject areas

    • Engineering(all)
    • Physics and Astronomy(all)

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