Fluorescence imaging of Escherichia coli on a rotating optical disk

Takayuki Shima, Hideyuki Shiramizu, Makoto Fujimaki, Chiaki Kuroda, Yoshimichi Ohki

    Research output: Contribution to journalArticle

    Abstract

    We constructed a fluorescence image from a rotating optical disk sample by aligning laser-scanned results based on positioning marks formed on the disk substrate. When Escherichia coli cells and 0.75-μm-diameter polystyrene beads were placed on the sample surface and a cell staining solution was used, fluorescence from only E. coli cells was observed in the obtained image. This indicates that E. coli cells can be distinguished from polystyrene beads, an abiotic substance, in one image. A promising utilization of this method is as a sensor for finding targets among contaminants on a large area.

    Original languageEnglish
    Article number088003
    JournalJapanese Journal of Applied Physics
    Volume57
    Issue number8
    DOIs
    Publication statusPublished - 2018 Aug 1

    Fingerprint

    optical disks
    Escherichia
    Escherichia coli
    Fluorescence
    Imaging techniques
    fluorescence
    Polystyrenes
    cells
    beads
    polystyrene
    staining
    Impurities
    positioning
    contaminants
    Lasers
    Sensors
    Substrates
    sensors
    lasers

    ASJC Scopus subject areas

    • Engineering(all)
    • Physics and Astronomy(all)

    Cite this

    Fluorescence imaging of Escherichia coli on a rotating optical disk. / Shima, Takayuki; Shiramizu, Hideyuki; Fujimaki, Makoto; Kuroda, Chiaki; Ohki, Yoshimichi.

    In: Japanese Journal of Applied Physics, Vol. 57, No. 8, 088003, 01.08.2018.

    Research output: Contribution to journalArticle

    Shima, Takayuki ; Shiramizu, Hideyuki ; Fujimaki, Makoto ; Kuroda, Chiaki ; Ohki, Yoshimichi. / Fluorescence imaging of Escherichia coli on a rotating optical disk. In: Japanese Journal of Applied Physics. 2018 ; Vol. 57, No. 8.
    @article{31111f3eaaac4b288030897f9a3e55da,
    title = "Fluorescence imaging of Escherichia coli on a rotating optical disk",
    abstract = "We constructed a fluorescence image from a rotating optical disk sample by aligning laser-scanned results based on positioning marks formed on the disk substrate. When Escherichia coli cells and 0.75-μm-diameter polystyrene beads were placed on the sample surface and a cell staining solution was used, fluorescence from only E. coli cells was observed in the obtained image. This indicates that E. coli cells can be distinguished from polystyrene beads, an abiotic substance, in one image. A promising utilization of this method is as a sensor for finding targets among contaminants on a large area.",
    author = "Takayuki Shima and Hideyuki Shiramizu and Makoto Fujimaki and Chiaki Kuroda and Yoshimichi Ohki",
    year = "2018",
    month = "8",
    day = "1",
    doi = "10.7567/JJAP.57.088003",
    language = "English",
    volume = "57",
    journal = "Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes",
    issn = "0021-4922",
    publisher = "Japan Society of Applied Physics",
    number = "8",

    }

    TY - JOUR

    T1 - Fluorescence imaging of Escherichia coli on a rotating optical disk

    AU - Shima, Takayuki

    AU - Shiramizu, Hideyuki

    AU - Fujimaki, Makoto

    AU - Kuroda, Chiaki

    AU - Ohki, Yoshimichi

    PY - 2018/8/1

    Y1 - 2018/8/1

    N2 - We constructed a fluorescence image from a rotating optical disk sample by aligning laser-scanned results based on positioning marks formed on the disk substrate. When Escherichia coli cells and 0.75-μm-diameter polystyrene beads were placed on the sample surface and a cell staining solution was used, fluorescence from only E. coli cells was observed in the obtained image. This indicates that E. coli cells can be distinguished from polystyrene beads, an abiotic substance, in one image. A promising utilization of this method is as a sensor for finding targets among contaminants on a large area.

    AB - We constructed a fluorescence image from a rotating optical disk sample by aligning laser-scanned results based on positioning marks formed on the disk substrate. When Escherichia coli cells and 0.75-μm-diameter polystyrene beads were placed on the sample surface and a cell staining solution was used, fluorescence from only E. coli cells was observed in the obtained image. This indicates that E. coli cells can be distinguished from polystyrene beads, an abiotic substance, in one image. A promising utilization of this method is as a sensor for finding targets among contaminants on a large area.

    UR - http://www.scopus.com/inward/record.url?scp=85050966668&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=85050966668&partnerID=8YFLogxK

    U2 - 10.7567/JJAP.57.088003

    DO - 10.7567/JJAP.57.088003

    M3 - Article

    AN - SCOPUS:85050966668

    VL - 57

    JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

    JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

    SN - 0021-4922

    IS - 8

    M1 - 088003

    ER -