We constructed a fluorescence image from a rotating optical disk sample by aligning laser-scanned results based on positioning marks formed on the disk substrate. When Escherichia coli cells and 0.75-μm-diameter polystyrene beads were placed on the sample surface and a cell staining solution was used, fluorescence from only E. coli cells was observed in the obtained image. This indicates that E. coli cells can be distinguished from polystyrene beads, an abiotic substance, in one image. A promising utilization of this method is as a sensor for finding targets among contaminants on a large area.
ASJC Scopus subject areas
- Physics and Astronomy(all)