Fluorescence microscopic investigation on morphological changes of giant multilamellar vesicles induced by amphiphilic additives

Taro Toyota, Hirotatsu Tsuha, Koji Yamada, Katsuto Takakura, Kenji Yasuda, Tadashi Sugawara

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Adding an artificial bolaamphiphile to a dispersion of giant multilamellar vesicles (GMVs) made of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) induced a cup-shaped deformation in GMVs accompanied by partial extrusion of the inner vesicle; thereafter, the deformed vesicles returned to their original shape. On the other hand, when the artificial bolaamphiphile together with a surfactant was added to the vesicular dispersion, these deformation and reformation dynamics were transmitted from the outer membranes in GMVs to the inner membranes until an intact inner vesicle was extruded out of the outer membrane. The microscopic aspects of these processes were investigated using amphiphiles tagged with individual fluorophores.

Original languageEnglish
Pages (from-to)1976-1981
Number of pages6
JournalLangmuir
Volume22
Issue number5
DOIs
Publication statusPublished - 2006 Feb 28
Externally publishedYes

Fingerprint

Fluorescence
membranes
Membranes
fluorescence
Amphiphiles
Fluorophores
Surface-Active Agents
Extrusion
Surface active agents
surfactants
bolaamphiphile
1-palmitoyl-2-oleoylphosphatidylcholine

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Fluorescence microscopic investigation on morphological changes of giant multilamellar vesicles induced by amphiphilic additives. / Toyota, Taro; Tsuha, Hirotatsu; Yamada, Koji; Takakura, Katsuto; Yasuda, Kenji; Sugawara, Tadashi.

In: Langmuir, Vol. 22, No. 5, 28.02.2006, p. 1976-1981.

Research output: Contribution to journalArticle

Toyota, Taro ; Tsuha, Hirotatsu ; Yamada, Koji ; Takakura, Katsuto ; Yasuda, Kenji ; Sugawara, Tadashi. / Fluorescence microscopic investigation on morphological changes of giant multilamellar vesicles induced by amphiphilic additives. In: Langmuir. 2006 ; Vol. 22, No. 5. pp. 1976-1981.
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