Fluorescence replication banding of frog chromosomes

I. Miura, H. Ohtani, M. Nakamura, K. Saitoh

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2 Citations (Scopus)

Abstract

To identify individual chromosomes of a frog karyotype by their fluorescence banding patterns, chromosomes were stained with actinomycin D and 4,6-diamidino-2-phenylindole (DAPI) after incorporation of BrdU during the late S-phase. The chromosomes of three Rana species which were selected for this study (R. ridibunda, R. lessonae and R. japonica) showed well-defined late replication bands. The fluorescence patterns obtained were the reverse of those produced by a 4Na-EDTA Giemsa-staining technique. Fluorescence patterns of the two water frog species (R. ridibunda and R. lessonae) were similar to each other, except for the different fluorescence of the centromeric heterochromatin, which gave extremely bright signals in R. ridibunda but no signal in R. lessonae. Experiments also showed differences between the fluorescence patterns of R. lessonae chromosome 13 in the Italian and Luxembourgian populations. These results show that the fluorescence replication banding using actinomycin D and DAPI is very effective in identifying individual frog chromosomes and detecting their structural changes.

Original languageEnglish
Pages (from-to)73-77
Number of pages5
JournalCellular and Molecular Life Sciences
Volume53
Issue number1
DOIs
Publication statusPublished - 1997
Externally publishedYes

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Keywords

  • Actinomycin D
  • DAPI
  • Frogs
  • Late replication banding

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

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