Fluorescence-signaling aptasensor for ATP and PDGF detection on functionalized diamond surface

A. Rahim Ruslinda; Y. Ishiyama; X. Wang; T. Kobayashi; H. Kawarada

doi:10.1149/2.083205jes

Fluorescence-signaling aptasensor for ATP and PDGF detection on functionalized diamond surface

A. Rahim Ruslinda^*, Y. Ishiyama, X. Wang, T. Kobayashi, H. Kawarada

^*Corresponding author for this work

School of Fundamental Science and Engineering

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

An aminated diamond-based aptasensor is presented for adenosine triphosphate (ATP) and platelet-derived growth factor (PDGF) detection. In this work, a signal-off detection method was employed to provide a sensitive, selective and reusable platform for the simple detection of PDGF and ATP. The detection limit of 0.2 μnM and 0.2 M of PDGF and ATP were achieved, respectively. It was also demonstrated in this study that the aptasensor is selective for PDGF and ATP over other biomolecules. In addition, the reusability of the aptasensor was confirmed by performing multiple cycles, which shows that diamond is a promising candidate as a material for biosensor applications. Moreover, this detection system does not require a target label and it is unaffected by non-specific binding.

Original language	English
Pages (from-to)	J182-J187
Journal	Journal of the Electrochemical Society
Volume	159
Issue number	5
DOIs	https://doi.org/10.1149/2.083205jes
Publication status	Published - 2012

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Renewable Energy, Sustainability and the Environment
Surfaces, Coatings and Films
Electrochemistry
Materials Chemistry

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abstract = "An aminated diamond-based aptasensor is presented for adenosine triphosphate (ATP) and platelet-derived growth factor (PDGF) detection. In this work, a signal-off detection method was employed to provide a sensitive, selective and reusable platform for the simple detection of PDGF and ATP. The detection limit of 0.2 μnM and 0.2 M of PDGF and ATP were achieved, respectively. It was also demonstrated in this study that the aptasensor is selective for PDGF and ATP over other biomolecules. In addition, the reusability of the aptasensor was confirmed by performing multiple cycles, which shows that diamond is a promising candidate as a material for biosensor applications. Moreover, this detection system does not require a target label and it is unaffected by non-specific binding.",

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T1 - Fluorescence-signaling aptasensor for ATP and PDGF detection on functionalized diamond surface

AU - Ruslinda, A. Rahim

AU - Ishiyama, Y.

AU - Wang, X.

AU - Kobayashi, T.

AU - Kawarada, H.

PY - 2012

Y1 - 2012

N2 - An aminated diamond-based aptasensor is presented for adenosine triphosphate (ATP) and platelet-derived growth factor (PDGF) detection. In this work, a signal-off detection method was employed to provide a sensitive, selective and reusable platform for the simple detection of PDGF and ATP. The detection limit of 0.2 μnM and 0.2 M of PDGF and ATP were achieved, respectively. It was also demonstrated in this study that the aptasensor is selective for PDGF and ATP over other biomolecules. In addition, the reusability of the aptasensor was confirmed by performing multiple cycles, which shows that diamond is a promising candidate as a material for biosensor applications. Moreover, this detection system does not require a target label and it is unaffected by non-specific binding.

AB - An aminated diamond-based aptasensor is presented for adenosine triphosphate (ATP) and platelet-derived growth factor (PDGF) detection. In this work, a signal-off detection method was employed to provide a sensitive, selective and reusable platform for the simple detection of PDGF and ATP. The detection limit of 0.2 μnM and 0.2 M of PDGF and ATP were achieved, respectively. It was also demonstrated in this study that the aptasensor is selective for PDGF and ATP over other biomolecules. In addition, the reusability of the aptasensor was confirmed by performing multiple cycles, which shows that diamond is a promising candidate as a material for biosensor applications. Moreover, this detection system does not require a target label and it is unaffected by non-specific binding.

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Fluorescence-signaling aptasensor for ATP and PDGF detection on functionalized diamond surface

Abstract

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