Fluorescence-signaling aptasensor for ATP and PDGF detection on functionalized diamond surface

A. Rahim Ruslinda, Y. Ishiyama, X. Wang, T. Kobayashi, Hiroshi Kawarada

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    An aminated diamond-based aptasensor is presented for adenosine triphosphate (ATP) and platelet-derived growth factor (PDGF) detection. In this work, a signal-off detection method was employed to provide a sensitive, selective and reusable platform for the simple detection of PDGF and ATP. The detection limit of 0.2 μnM and 0.2 M of PDGF and ATP were achieved, respectively. It was also demonstrated in this study that the aptasensor is selective for PDGF and ATP over other biomolecules. In addition, the reusability of the aptasensor was confirmed by performing multiple cycles, which shows that diamond is a promising candidate as a material for biosensor applications. Moreover, this detection system does not require a target label and it is unaffected by non-specific binding.

    Original languageEnglish
    JournalJournal of the Electrochemical Society
    Volume159
    Issue number5
    DOIs
    Publication statusPublished - 2012

    Fingerprint

    Diamond
    adenosine triphosphate
    Platelet-Derived Growth Factor
    Platelets
    platelets
    Diamonds
    Adenosine Triphosphate
    Fluorescence
    diamonds
    fluorescence
    Reusability
    Biomolecules
    Biosensors
    Labels
    bioinstrumentation
    platforms
    Intercellular Signaling Peptides and Proteins
    cycles

    ASJC Scopus subject areas

    • Electrochemistry
    • Electronic, Optical and Magnetic Materials
    • Materials Chemistry
    • Surfaces, Coatings and Films
    • Renewable Energy, Sustainability and the Environment
    • Condensed Matter Physics

    Cite this

    Fluorescence-signaling aptasensor for ATP and PDGF detection on functionalized diamond surface. / Ruslinda, A. Rahim; Ishiyama, Y.; Wang, X.; Kobayashi, T.; Kawarada, Hiroshi.

    In: Journal of the Electrochemical Society, Vol. 159, No. 5, 2012.

    Research output: Contribution to journalArticle

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    AU - Ishiyama, Y.

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    AU - Kobayashi, T.

    AU - Kawarada, Hiroshi

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