Fluorescence-signaling aptasensor for ATP and PDGF detection on functionalized diamond surface

A. Rahim Ruslinda, Y. Ishiyama, X. Wang, T. Kobayashi, H. Kawarada

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

An aminated diamond-based aptasensor is presented for adenosine triphosphate (ATP) and platelet-derived growth factor (PDGF) detection. In this work, a signal-off detection method was employed to provide a sensitive, selective and reusable platform for the simple detection of PDGF and ATP. The detection limit of 0.2 μnM and 0.2 M of PDGF and ATP were achieved, respectively. It was also demonstrated in this study that the aptasensor is selective for PDGF and ATP over other biomolecules. In addition, the reusability of the aptasensor was confirmed by performing multiple cycles, which shows that diamond is a promising candidate as a material for biosensor applications. Moreover, this detection system does not require a target label and it is unaffected by non-specific binding.

Original languageEnglish
Pages (from-to)J182-J187
JournalJournal of the Electrochemical Society
Volume159
Issue number5
DOIs
Publication statusPublished - 2012 Apr 9

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Renewable Energy, Sustainability and the Environment
  • Surfaces, Coatings and Films
  • Electrochemistry
  • Materials Chemistry

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