Fluorogenic probe triggered by reduction for nucleic acids sensing.

Kazuhiro Furukawa, Hiroshi Abe, Jin Wang, Kazuma Oki, Miwako Uda, Satoshi Tsuneda, Yoshihiro Ito

Research output: Contribution to journalArticle

Abstract

A reduction-triggered fluorescence probe with a new fluorogenic compound derivatized from rhodamine 110 was developed for sensing oligonucleotides. The chemistry to activate the compound involves the reaction between the azide group of rhodamine derivatives and reducing reagents, with the fluorescence signal appearing after reduction of the azide group. The reaction proceeds under biological conditions to produce fluorescence signal within 10-20 min in the presence of target DNA or RNA. The probes were successfully applied to the detection of oligonucleotides in solution and endogenous RNA in bacterial cells.

Original languageEnglish
Pages (from-to)353-354
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number52
Publication statusPublished - 2008
Externally publishedYes

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ASJC Scopus subject areas

  • Medicine(all)

Cite this

Furukawa, K., Abe, H., Wang, J., Oki, K., Uda, M., Tsuneda, S., & Ito, Y. (2008). Fluorogenic probe triggered by reduction for nucleic acids sensing. Nucleic acids symposium series (2004), (52), 353-354.