Fluorogenic probe triggered by reduction for nucleic acids sensing.

Kazuhiro Furukawa; Hiroshi Abe; Jin Wang; Kazuma Oki; Miwako Uda; Satoshi Tsuneda; Yoshihiro Ito

Fluorogenic probe triggered by reduction for nucleic acids sensing.

Kazuhiro Furukawa^*, Hiroshi Abe, Jin Wang, Kazuma Oki, Miwako Uda, Satoshi Tsuneda, Yoshihiro Ito

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A reduction-triggered fluorescence probe with a new fluorogenic compound derivatized from rhodamine 110 was developed for sensing oligonucleotides. The chemistry to activate the compound involves the reaction between the azide group of rhodamine derivatives and reducing reagents, with the fluorescence signal appearing after reduction of the azide group. The reaction proceeds under biological conditions to produce fluorescence signal within 10-20 min in the presence of target DNA or RNA. The probes were successfully applied to the detection of oligonucleotides in solution and endogenous RNA in bacterial cells.

Original language	English
Pages (from-to)	353-354
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	52
Publication status	Published - 2008 Dec 1
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

Cite this

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title = "Fluorogenic probe triggered by reduction for nucleic acids sensing.",

abstract = "A reduction-triggered fluorescence probe with a new fluorogenic compound derivatized from rhodamine 110 was developed for sensing oligonucleotides. The chemistry to activate the compound involves the reaction between the azide group of rhodamine derivatives and reducing reagents, with the fluorescence signal appearing after reduction of the azide group. The reaction proceeds under biological conditions to produce fluorescence signal within 10-20 min in the presence of target DNA or RNA. The probes were successfully applied to the detection of oligonucleotides in solution and endogenous RNA in bacterial cells.",

author = "Kazuhiro Furukawa and Hiroshi Abe and Jin Wang and Kazuma Oki and Miwako Uda and Satoshi Tsuneda and Yoshihiro Ito",

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T1 - Fluorogenic probe triggered by reduction for nucleic acids sensing.

AU - Furukawa, Kazuhiro

AU - Abe, Hiroshi

AU - Wang, Jin

AU - Oki, Kazuma

AU - Uda, Miwako

AU - Tsuneda, Satoshi

AU - Ito, Yoshihiro

PY - 2008/12/1

Y1 - 2008/12/1

N2 - A reduction-triggered fluorescence probe with a new fluorogenic compound derivatized from rhodamine 110 was developed for sensing oligonucleotides. The chemistry to activate the compound involves the reaction between the azide group of rhodamine derivatives and reducing reagents, with the fluorescence signal appearing after reduction of the azide group. The reaction proceeds under biological conditions to produce fluorescence signal within 10-20 min in the presence of target DNA or RNA. The probes were successfully applied to the detection of oligonucleotides in solution and endogenous RNA in bacterial cells.

AB - A reduction-triggered fluorescence probe with a new fluorogenic compound derivatized from rhodamine 110 was developed for sensing oligonucleotides. The chemistry to activate the compound involves the reaction between the azide group of rhodamine derivatives and reducing reagents, with the fluorescence signal appearing after reduction of the azide group. The reaction proceeds under biological conditions to produce fluorescence signal within 10-20 min in the presence of target DNA or RNA. The probes were successfully applied to the detection of oligonucleotides in solution and endogenous RNA in bacterial cells.

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M3 - Article

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AN - SCOPUS:78649434236

SN - 1746-8272

SP - 353

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JO - Nucleic acids symposium series (2004)

JF - Nucleic acids symposium series (2004)

IS - 52

ER -

Fluorogenic probe triggered by reduction for nucleic acids sensing.

Abstract

ASJC Scopus subject areas

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