Fluoroimmunoassay for the determination of allergen using bacterial magnetic particles

Noriyuki Nakamura, Tadashi Matsunaga

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have developed a novel fluoroimmunoassay method using bacterial magnetic particles for the determination of allergen. Fluorescein isothiocyanate (FITC) conjugated IgE antibody was immobilized onto bacterial magnetic particles. The decrease of fluorescence intensity by aggregation of FITC-labeled bacterial magnetic particle conjugates was determined by a fluorescence spectrophotometer. The relative fluorescence intensity decreased with increasing allergen concentration. A linear relationship was obtained between the relative fluorescence intensity and allergen concentration in the range of 0.1 100 ng/ml. This method may be carried out across a wider concentration range having a linear response than when artificial magnetite particles are used.

Original languageEnglish
Pages (from-to)899-902
Number of pages4
JournalAnalytical Sciences
Volume7
Issue numberSupplement
DOIs
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Allergens
Fluorescence
Fluorescein
Ferrosoferric Oxide
Spectrophotometers
Immunoglobulin E
Agglomeration
Antibodies
isothiocyanic acid

Keywords

  • allergen
  • bacterial magnetic particles
  • fluoroimmunoassay
  • IgE

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Fluoroimmunoassay for the determination of allergen using bacterial magnetic particles. / Nakamura, Noriyuki; Matsunaga, Tadashi.

In: Analytical Sciences, Vol. 7, No. Supplement, 1991, p. 899-902.

Research output: Contribution to journalArticle

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