Flux characteristics of cell culture medium in rectangular microchannels

Zhonggang Feng, Shuhei Fukuda, Michio Yokoyama, Tatsuo Kitajima, Takao Nakamura, Mitsuo Umezu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Rectangular microchannels 50 μm high and 30, 40, 50, 60, or 70 μm wide were fabricated by adjusting the width of a gap cut in a polyethylene sheet 50 μm thick and sandwiching the sheet between an acrylic plate and a glass plate. Flux in the microchannels was measured under three different inner surface conditions: uncoated, albumin-coated, and confluent growth of rat fibroblasts on the bottom of the microchannels. The normalized flux in microchannels with cultured fibroblasts or albumin coating was significantly larger than that in the uncoated channels. The experimental data for all microchannels deviated from that predicted by classical hydrodynamic theory. At small aspect ratio the flux in the microchannels was larger than that predicted theoretically, whereas it became smaller at large aspect ratio. The aspect ratio rather than Reynolds number is the correct property for predicting the variation of the normalized friction factor. We postulate that two counteracting effects, rotation of large molecules and slip velocity at the corners of the microchannels, are responsible for the deviation. From these results we conclude that albumin coating should be carried out in the same way as when fabricating our integrating cell-culture system. The outcomes of this study are not only important for the design of our culture system, but also quite informative for general microfluidics.

Original languageEnglish
Pages (from-to)238-244
Number of pages7
JournalJournal of Artificial Organs
Volume14
Issue number3
DOIs
Publication statusPublished - 2011 Sep 1

Keywords

  • Albumin coating
  • Aspect ratio
  • Cultured fibroblasts
  • Flux
  • Normalized friction factor
  • Rectangular microchannels
  • Reynolds number

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering
  • Cardiology and Cardiovascular Medicine

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