Flux characteristics of cell culture medium in rectangular microchannels

Zhonggang Feng, Shuhei Fukuda, Michio Yokoyama, Tatsuo Kitajima, Takao Nakamura, Mitsuo Umezu

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Rectangular microchannels 50 μm high and 30, 40, 50, 60, or 70 μm wide were fabricated by adjusting the width of a gap cut in a polyethylene sheet 50 μm thick and sandwiching the sheet between an acrylic plate and a glass plate. Flux in the microchannels was measured under three different inner surface conditions: uncoated, albumin-coated, and confluent growth of rat fibroblasts on the bottom of the microchannels. The normalized flux in microchannels with cultured fibroblasts or albumin coating was significantly larger than that in the uncoated channels. The experimental data for all microchannels deviated from that predicted by classical hydrodynamic theory. At small aspect ratio the flux in the microchannels was larger than that predicted theoretically, whereas it became smaller at large aspect ratio. The aspect ratio rather than Reynolds number is the correct property for predicting the variation of the normalized friction factor. We postulate that two counteracting effects, rotation of large molecules and slip velocity at the corners of the microchannels, are responsible for the deviation. From these results we conclude that albumin coating should be carried out in the same way as when fabricating our integrating cell-culture system. The outcomes of this study are not only important for the design of our culture system, but also quite informative for general microfluidics.

    Original languageEnglish
    Pages (from-to)238-244
    Number of pages7
    JournalJournal of Artificial Organs
    Volume14
    Issue number3
    DOIs
    Publication statusPublished - 2011 Sep

    Fingerprint

    Microchannels
    Cell culture
    Culture Media
    Albumins
    Cell Culture Techniques
    Fluxes
    Fibroblasts
    Microfluidics
    Friction
    Aspect ratio
    Polyethylene
    Hydrodynamics
    Glass
    Outcome Assessment (Health Care)
    Coatings
    Growth
    Acrylics
    Polyethylenes
    Rats
    Reynolds number

    Keywords

    • Albumin coating
    • Aspect ratio
    • Cultured fibroblasts
    • Flux
    • Normalized friction factor
    • Rectangular microchannels
    • Reynolds number

    ASJC Scopus subject areas

    • Biomaterials
    • Biomedical Engineering
    • Cardiology and Cardiovascular Medicine
    • Medicine (miscellaneous)

    Cite this

    Flux characteristics of cell culture medium in rectangular microchannels. / Feng, Zhonggang; Fukuda, Shuhei; Yokoyama, Michio; Kitajima, Tatsuo; Nakamura, Takao; Umezu, Mitsuo.

    In: Journal of Artificial Organs, Vol. 14, No. 3, 09.2011, p. 238-244.

    Research output: Contribution to journalArticle

    Feng, Zhonggang ; Fukuda, Shuhei ; Yokoyama, Michio ; Kitajima, Tatsuo ; Nakamura, Takao ; Umezu, Mitsuo. / Flux characteristics of cell culture medium in rectangular microchannels. In: Journal of Artificial Organs. 2011 ; Vol. 14, No. 3. pp. 238-244.
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