Folate-deficiency induced cell-specific changes in the distribution of lymphocytes and granulocytes in rats

Ikumi Abe, Ken Shirato, Yoko Hashizume, Ryosuke Mitsuhashi, Ayumu Kobayashi, Chikako Shiono, Shogo Sato, Kaoru Tachiyashiki, Kazuhiko Imaizumi

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Objective: Folate (vitamin B9) plays key roles in cell growth and proliferation through regulating the synthesis and stabilization of DNA and RNA, and its deficiency leads to lymphocytopenia and granulocytopenia. However, precisely how folate deficiency affects the distribution of a variety of white blood cell subsets, including the minor population of basophils, and the cell specificity of the effects remain unclear. Therefore, we examined the effects of a folate-deficient diet on the circulating number of lymphocyte subsets [T-lymphocytes, B-lymphocytes, and natural killer (NK) cells] and granulocyte subsets (neutrophils, eosinophils, and basophils) in rats. Methods: Rats were divided into two groups, with one receiving the folate-deficient diet (FAD group) and the other a control diet (CON group). All rats were pair-fed for 8 weeks. Results: Plasma folate level was dramatically lower in the FAD group than in the CON group, and the level of homocysteine in the plasma, a predictor of folate deficiency was significantly higher in the FAD group than in the CON group. The number of T-lymphocytes, B-lymphocytes, and NK cells was significantly lower in the FAD group than in the CON group by 0.73-, 0.49-, and 0.70-fold, respectively, indicating that B-lymphocytes are more sensitive to folate deficiency than the other lymphocyte subsets. As expected, the number of neutrophils and eosinophils was significantly lower in the FAD group than in the CON group. However, the number of basophils, the least common type of granulocyte, showed transiently an increasing tendency in the FAD group as compared with the CON group. Conclusion: These results suggest that folate deficiency induces lymphocytopenia and granulocytopenia in a cell-specific manner.

    Original languageEnglish
    Pages (from-to)78-84
    Number of pages7
    JournalEnvironmental Health and Preventive Medicine
    Volume18
    Issue number1
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    Folic Acid
    Granulocytes
    Flavin-Adenine Dinucleotide
    Lymphocytes
    Basophils
    Lymphopenia
    Agranulocytosis
    B-Lymphocytes
    T-Lymphocyte Subsets
    Diet
    Eosinophils
    Natural Killer Cells
    Neutrophils
    Lymphocyte Subsets
    Homocysteine
    Leukocytes
    Cell Proliferation
    RNA
    T-Lymphocytes
    DNA

    Keywords

    • Dietary folate-deficiency
    • Granulocyte subsets
    • Lymphocyte subsets

    ASJC Scopus subject areas

    • Public Health, Environmental and Occupational Health

    Cite this

    Folate-deficiency induced cell-specific changes in the distribution of lymphocytes and granulocytes in rats. / Abe, Ikumi; Shirato, Ken; Hashizume, Yoko; Mitsuhashi, Ryosuke; Kobayashi, Ayumu; Shiono, Chikako; Sato, Shogo; Tachiyashiki, Kaoru; Imaizumi, Kazuhiko.

    In: Environmental Health and Preventive Medicine, Vol. 18, No. 1, 2013, p. 78-84.

    Research output: Contribution to journalArticle

    Abe, I, Shirato, K, Hashizume, Y, Mitsuhashi, R, Kobayashi, A, Shiono, C, Sato, S, Tachiyashiki, K & Imaizumi, K 2013, 'Folate-deficiency induced cell-specific changes in the distribution of lymphocytes and granulocytes in rats', Environmental Health and Preventive Medicine, vol. 18, no. 1, pp. 78-84. https://doi.org/10.1007/s12199-012-0286-6
    Abe, Ikumi ; Shirato, Ken ; Hashizume, Yoko ; Mitsuhashi, Ryosuke ; Kobayashi, Ayumu ; Shiono, Chikako ; Sato, Shogo ; Tachiyashiki, Kaoru ; Imaizumi, Kazuhiko. / Folate-deficiency induced cell-specific changes in the distribution of lymphocytes and granulocytes in rats. In: Environmental Health and Preventive Medicine. 2013 ; Vol. 18, No. 1. pp. 78-84.
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    AU - Shirato, Ken

    AU - Hashizume, Yoko

    AU - Mitsuhashi, Ryosuke

    AU - Kobayashi, Ayumu

    AU - Shiono, Chikako

    AU - Sato, Shogo

    AU - Tachiyashiki, Kaoru

    AU - Imaizumi, Kazuhiko

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