Food restriction causes low bone strength and microarchitectural deterioration in exercised growing male rats

Satoshi Hattori, Jong Hoon Park, Umon Agata, Masaya Oda, Michito Higano, Yuki Aikawa, Takayuki Akimoto, Yoshiharu Nabekura, Hideyuki Yamato, Ikuko Ezawa, Naomi Omi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The pathogenesis of bone disorders in young male athletes has not been well understood. We hypothesized that bone fragility is caused by low energy availability, due to insufficient food intake and excessive exercise energy expenditure in young male athletes. To examine this hypothesis, we investigated the influence of food restriction on bone strength and bone morphology in exercised growing male rats, using three-point bending test, dual-energy X-ray absormetry, and micro-computed tomography. Four-week-old male Sprague-Dawley rats were divided randomly into the following groups: the control (Con) group, exercise (Ex) group, food restriction (R) group, and food restriction plus exercise (REx) group after a 1-wk acclimatization period. Thirty-percent food restriction in the R and REx groups was carried out in comparison with that in the Con group. Voluntary running exercise was performed in the Ex and REx groups. The experimental period lasted 13 wk. At the end-point of this experiment, the bone strength of the femurs and tibial BMD in the REx group were significantly lower than those in the Con group. Moreover, trabecular bone volume and cortical bone volume in the REx group were also significantly lower than those in the Con group. These findings indicate that food restriction causes low bone strength and microarchitectural deterioration in exercised growing male rats.

Original languageEnglish
Pages (from-to)35-42
Number of pages8
JournalJournal of Nutritional Science and Vitaminology
Volume60
Issue number1
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Exercise
Bone and Bones
Food
Control Groups
Athletes
X-Ray Microtomography
Acclimatization
Running
Femur
Energy Metabolism
Sprague Dawley Rats
Eating

Keywords

  • Bone mineral density
  • Bone morphology
  • Bone strength
  • Low energy availability
  • Voluntary running exercise

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Medicine(all)

Cite this

Food restriction causes low bone strength and microarchitectural deterioration in exercised growing male rats. / Hattori, Satoshi; Park, Jong Hoon; Agata, Umon; Oda, Masaya; Higano, Michito; Aikawa, Yuki; Akimoto, Takayuki; Nabekura, Yoshiharu; Yamato, Hideyuki; Ezawa, Ikuko; Omi, Naomi.

In: Journal of Nutritional Science and Vitaminology, Vol. 60, No. 1, 2014, p. 35-42.

Research output: Contribution to journalArticle

Hattori, S, Park, JH, Agata, U, Oda, M, Higano, M, Aikawa, Y, Akimoto, T, Nabekura, Y, Yamato, H, Ezawa, I & Omi, N 2014, 'Food restriction causes low bone strength and microarchitectural deterioration in exercised growing male rats', Journal of Nutritional Science and Vitaminology, vol. 60, no. 1, pp. 35-42. https://doi.org/10.3177/jnsv.60.35
Hattori, Satoshi ; Park, Jong Hoon ; Agata, Umon ; Oda, Masaya ; Higano, Michito ; Aikawa, Yuki ; Akimoto, Takayuki ; Nabekura, Yoshiharu ; Yamato, Hideyuki ; Ezawa, Ikuko ; Omi, Naomi. / Food restriction causes low bone strength and microarchitectural deterioration in exercised growing male rats. In: Journal of Nutritional Science and Vitaminology. 2014 ; Vol. 60, No. 1. pp. 35-42.
@article{e4ffda26b99d41a38f2397e6dc8d6d94,
title = "Food restriction causes low bone strength and microarchitectural deterioration in exercised growing male rats",
abstract = "The pathogenesis of bone disorders in young male athletes has not been well understood. We hypothesized that bone fragility is caused by low energy availability, due to insufficient food intake and excessive exercise energy expenditure in young male athletes. To examine this hypothesis, we investigated the influence of food restriction on bone strength and bone morphology in exercised growing male rats, using three-point bending test, dual-energy X-ray absormetry, and micro-computed tomography. Four-week-old male Sprague-Dawley rats were divided randomly into the following groups: the control (Con) group, exercise (Ex) group, food restriction (R) group, and food restriction plus exercise (REx) group after a 1-wk acclimatization period. Thirty-percent food restriction in the R and REx groups was carried out in comparison with that in the Con group. Voluntary running exercise was performed in the Ex and REx groups. The experimental period lasted 13 wk. At the end-point of this experiment, the bone strength of the femurs and tibial BMD in the REx group were significantly lower than those in the Con group. Moreover, trabecular bone volume and cortical bone volume in the REx group were also significantly lower than those in the Con group. These findings indicate that food restriction causes low bone strength and microarchitectural deterioration in exercised growing male rats.",
keywords = "Bone mineral density, Bone morphology, Bone strength, Low energy availability, Voluntary running exercise",
author = "Satoshi Hattori and Park, {Jong Hoon} and Umon Agata and Masaya Oda and Michito Higano and Yuki Aikawa and Takayuki Akimoto and Yoshiharu Nabekura and Hideyuki Yamato and Ikuko Ezawa and Naomi Omi",
year = "2014",
doi = "10.3177/jnsv.60.35",
language = "English",
volume = "60",
pages = "35--42",
journal = "Journal of Nutritional Science and Vitaminology",
issn = "0301-4800",
publisher = "Center for Academic Publications Japan",
number = "1",

}

TY - JOUR

T1 - Food restriction causes low bone strength and microarchitectural deterioration in exercised growing male rats

AU - Hattori, Satoshi

AU - Park, Jong Hoon

AU - Agata, Umon

AU - Oda, Masaya

AU - Higano, Michito

AU - Aikawa, Yuki

AU - Akimoto, Takayuki

AU - Nabekura, Yoshiharu

AU - Yamato, Hideyuki

AU - Ezawa, Ikuko

AU - Omi, Naomi

PY - 2014

Y1 - 2014

N2 - The pathogenesis of bone disorders in young male athletes has not been well understood. We hypothesized that bone fragility is caused by low energy availability, due to insufficient food intake and excessive exercise energy expenditure in young male athletes. To examine this hypothesis, we investigated the influence of food restriction on bone strength and bone morphology in exercised growing male rats, using three-point bending test, dual-energy X-ray absormetry, and micro-computed tomography. Four-week-old male Sprague-Dawley rats were divided randomly into the following groups: the control (Con) group, exercise (Ex) group, food restriction (R) group, and food restriction plus exercise (REx) group after a 1-wk acclimatization period. Thirty-percent food restriction in the R and REx groups was carried out in comparison with that in the Con group. Voluntary running exercise was performed in the Ex and REx groups. The experimental period lasted 13 wk. At the end-point of this experiment, the bone strength of the femurs and tibial BMD in the REx group were significantly lower than those in the Con group. Moreover, trabecular bone volume and cortical bone volume in the REx group were also significantly lower than those in the Con group. These findings indicate that food restriction causes low bone strength and microarchitectural deterioration in exercised growing male rats.

AB - The pathogenesis of bone disorders in young male athletes has not been well understood. We hypothesized that bone fragility is caused by low energy availability, due to insufficient food intake and excessive exercise energy expenditure in young male athletes. To examine this hypothesis, we investigated the influence of food restriction on bone strength and bone morphology in exercised growing male rats, using three-point bending test, dual-energy X-ray absormetry, and micro-computed tomography. Four-week-old male Sprague-Dawley rats were divided randomly into the following groups: the control (Con) group, exercise (Ex) group, food restriction (R) group, and food restriction plus exercise (REx) group after a 1-wk acclimatization period. Thirty-percent food restriction in the R and REx groups was carried out in comparison with that in the Con group. Voluntary running exercise was performed in the Ex and REx groups. The experimental period lasted 13 wk. At the end-point of this experiment, the bone strength of the femurs and tibial BMD in the REx group were significantly lower than those in the Con group. Moreover, trabecular bone volume and cortical bone volume in the REx group were also significantly lower than those in the Con group. These findings indicate that food restriction causes low bone strength and microarchitectural deterioration in exercised growing male rats.

KW - Bone mineral density

KW - Bone morphology

KW - Bone strength

KW - Low energy availability

KW - Voluntary running exercise

UR - http://www.scopus.com/inward/record.url?scp=84899434002&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899434002&partnerID=8YFLogxK

U2 - 10.3177/jnsv.60.35

DO - 10.3177/jnsv.60.35

M3 - Article

VL - 60

SP - 35

EP - 42

JO - Journal of Nutritional Science and Vitaminology

JF - Journal of Nutritional Science and Vitaminology

SN - 0301-4800

IS - 1

ER -