ForC, a novel type of formin family protein lacking an FH1 domain, is involved in multicellular development in Dictyostelium discoideum

Chikako Kitayama, Taro Q.P. Uyeda

Research output: Contribution to journalReview articlepeer-review

31 Citations (Scopus)

Abstract

Formins are highly conserved regulators of cytoskeletal organization and share three regions of homology: the FH1, FH2 and FH3 domains. Of the nine known formin genes or pseudogenes carried by Dictyostelium, forC is novel in that it lacks an FH1 domain. Mutant Dictyostelium lacking forC (ΔforC) grew normally during the vegetative phase and, when starved, migrated normally and formed tight aggregates. Subsequently, however, ΔforC cells made aberrant fruiting bodies with short stalks and sori that remained unlifted. ΔforC aggregates were also unable to migrate as slugs, suggesting forC is involved in mediating cell movement during multicellular stages of Dictyostelium development. Consistent with this idea, expression of forC was increased significantly in aggregates of wild-type cells. GFP-ForC expressed in ΔforC cells was localized at the crowns, which are macropinocytotic structures rich in F-actin, suggesting that, like other formin isoforms, ForC functions in close relation with the actin cytoskeleton. Truncation analysis of GFP-ForC revealed that the FH3 domain is required for ForC localization; moreover, localization of a truncated GFP-ForC mutant at the site of contacts between cells on substrates and along the cortex of cells within a multicellular culminant suggests that ForC is involved in the local actin cytoskeletal reorganization mediating cell-cell adhesion.

Original languageEnglish
Pages (from-to)711-723
Number of pages13
JournalJournal of Cell Science
Volume116
Issue number4
DOIs
Publication statusPublished - 2003 Feb 15

Keywords

  • Actin
  • Cellular slime mold
  • Culmination
  • Morphogenesis
  • Profilin
  • Slug

ASJC Scopus subject areas

  • Cell Biology

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