Foreign gene expression in marine cyanobacteria under pseudo-continuous culture

Koji Sode, Masahiro Tatara, Naoaki Hatano, Tadashi Matsunaga

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Foreign gene expression in a marine cyanobacterium, Synechococcus NKBG 15041c, has been carried out in both batch and pseudo-continuous culture, using chloramphenicol acetyltransferase (CAT) as a model peptide and the broad host range vector pKT230. CAT has been successfully expressed in marine cyanobacteria under kanamycin resistance gene promoter. Pseudo-continuous culture of these recombinant marine cyanobacteria has been done by varying the dilution rate. At a dilution rate between 0.01 to 0.02 h-1, highest productivity was achieved. Under these conditions, long-term pseudo-continuous culture of recombinant marine cyanobacteria was achieved for more than 600 h with CAT productivity 18-fold greater than that observed in batch culture.

Original languageEnglish
Pages (from-to)243-248
Number of pages6
JournalJournal of Biotechnology
Volume33
Issue number3
DOIs
Publication statusPublished - 1994 Apr 15
Externally publishedYes

Keywords

  • Broad host range vector
  • Chloramphenicol acetyl-transferase
  • Foreign gene expression
  • Marine cyanobacteria
  • Pseudo-continuous culture

ASJC Scopus subject areas

  • Biotechnology

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