Formation and characteristics of nitrifying biofilm on a membrane modified with positively-charged polymer chains

Kazuaki Hibiya, Satoshi Tsuneda, Akira Hirata

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    Nitrifying bacteria hardly adhered to the surface of supporting materials in a fluidized bed bioreactor containing only inorganic compounds. The adhesivity of nitrifying bacteria was compared, between an unmodified membrane and a membrane modified with diethylamino groups, using the grafting method. The result showed that the diethylamino-group-grafted membrane successfully increased the adhesivity of nitrifying bacteria, which might be due to particular properties of the grafted membrane surface, such as positive charge and soft polymer chains. Moreover, the thickness of the biofilm exceeded 100 μm after incubation for 150 days in a continuously fed fluidized bed bioreactor. The results of fluorescence in situ hybridization (FISH) confirmed that the biofilm consisted mostly of ammonia-oxidizing bacteria. Moreover, the biofilm exhibited a much higher nitrification rate per unit area than other biofilms, which was confirmed by the analysis of the concentration profile of ammonium-nitrogen inside the biofilm using a microelectrode that we originally fabricated. Copyright (C) 2000 Elsevier Science B.V.

    Original languageEnglish
    Pages (from-to)105-112
    Number of pages8
    JournalColloids and Surfaces B: Biointerfaces
    Volume18
    Issue number2
    DOIs
    Publication statusPublished - 2000 Aug

    Fingerprint

    biofilms
    Biofilms
    Polymers
    bacteria
    membranes
    Membranes
    Bacteria
    polymers
    bioreactors
    Bioreactors
    Fluidized beds
    beds
    Nitrification
    Inorganic compounds
    inorganic compounds
    Microelectrodes
    Fluorescence In Situ Hybridization
    Ammonium Compounds
    Ammonia
    ammonia

    Keywords

    • Bacterial adhesivity
    • Grafting method
    • Inorganic wastewater
    • Microelectrode
    • Nitrifying bacteria

    ASJC Scopus subject areas

    • Biotechnology
    • Colloid and Surface Chemistry
    • Physical and Theoretical Chemistry
    • Surfaces and Interfaces

    Cite this

    Formation and characteristics of nitrifying biofilm on a membrane modified with positively-charged polymer chains. / Hibiya, Kazuaki; Tsuneda, Satoshi; Hirata, Akira.

    In: Colloids and Surfaces B: Biointerfaces, Vol. 18, No. 2, 08.2000, p. 105-112.

    Research output: Contribution to journalArticle

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