Formation and intracellular movement of cytoplasmic bodies of the tomato mosaic virus 126-kDa replication protein in association with the viral movement protein

Nobumitsu Sasaki, Ryuki Shishikura, Hiroshi Nyunoya

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

During plant infection by tobamoviruses such as Tomato mosaic virus (ToMV), viral replication complexes containing a viral 126-kDa replicase component (126K) are generated, temporarily in association with a viral movement protein (MP) that is indispensable for viral intercellular movement. The tobamovirus 126K protein has been shown to play an important role not only in replication but also in intercellular transport of viral RNAs. Transient expression of the 126K protein has recently been reported to result in formation of cytoplasmic bodies with shapes similar to those of the replication complexes. In this study, to investigate the roles of ToMV MP in the formation, localization, and dynamics of cytoplasmic bodies of the 126K protein, we carried out confocal microscopy studies of fluorescently tagged 126K and MP that were transiently and simultaneously expressed in Nicotiana benthamiana. Without MP expression, 126K was evenly distributed in the cytoplasm, with occasional formation of small 126K bodies. These small 126K bodies moved in the cytoplasmic stream and along the endoplasmic reticulum network. In contrast, co-expression of MP markedly promoted the formation of larger MP-associated 126K bodies, which were generally static but moved occasionally in the cytoplasmic stream. In addition, we found that small, mobile 126K bodies were captured at the terminal part of MP-derived filamentous structures, leading to the formation of 126K-MP complexes and their translocation to another cellular site.

Original languageEnglish
Pages (from-to)272-281
Number of pages10
JournalJournal of General Plant Pathology
Volume80
Issue number3
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Tomato mosaic virus
proteins
Tobamovirus
protein synthesis
Nicotiana benthamiana
body protein
virus replication
endoplasmic reticulum
cytoplasm
RNA

Keywords

  • Cytoplasmic body
  • Intracellular movement
  • Movement protein
  • Replicase
  • Tomato mosaic virus

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

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title = "Formation and intracellular movement of cytoplasmic bodies of the tomato mosaic virus 126-kDa replication protein in association with the viral movement protein",
abstract = "During plant infection by tobamoviruses such as Tomato mosaic virus (ToMV), viral replication complexes containing a viral 126-kDa replicase component (126K) are generated, temporarily in association with a viral movement protein (MP) that is indispensable for viral intercellular movement. The tobamovirus 126K protein has been shown to play an important role not only in replication but also in intercellular transport of viral RNAs. Transient expression of the 126K protein has recently been reported to result in formation of cytoplasmic bodies with shapes similar to those of the replication complexes. In this study, to investigate the roles of ToMV MP in the formation, localization, and dynamics of cytoplasmic bodies of the 126K protein, we carried out confocal microscopy studies of fluorescently tagged 126K and MP that were transiently and simultaneously expressed in Nicotiana benthamiana. Without MP expression, 126K was evenly distributed in the cytoplasm, with occasional formation of small 126K bodies. These small 126K bodies moved in the cytoplasmic stream and along the endoplasmic reticulum network. In contrast, co-expression of MP markedly promoted the formation of larger MP-associated 126K bodies, which were generally static but moved occasionally in the cytoplasmic stream. In addition, we found that small, mobile 126K bodies were captured at the terminal part of MP-derived filamentous structures, leading to the formation of 126K-MP complexes and their translocation to another cellular site.",
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author = "Nobumitsu Sasaki and Ryuki Shishikura and Hiroshi Nyunoya",
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T1 - Formation and intracellular movement of cytoplasmic bodies of the tomato mosaic virus 126-kDa replication protein in association with the viral movement protein

AU - Sasaki, Nobumitsu

AU - Shishikura, Ryuki

AU - Nyunoya, Hiroshi

PY - 2014

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AB - During plant infection by tobamoviruses such as Tomato mosaic virus (ToMV), viral replication complexes containing a viral 126-kDa replicase component (126K) are generated, temporarily in association with a viral movement protein (MP) that is indispensable for viral intercellular movement. The tobamovirus 126K protein has been shown to play an important role not only in replication but also in intercellular transport of viral RNAs. Transient expression of the 126K protein has recently been reported to result in formation of cytoplasmic bodies with shapes similar to those of the replication complexes. In this study, to investigate the roles of ToMV MP in the formation, localization, and dynamics of cytoplasmic bodies of the 126K protein, we carried out confocal microscopy studies of fluorescently tagged 126K and MP that were transiently and simultaneously expressed in Nicotiana benthamiana. Without MP expression, 126K was evenly distributed in the cytoplasm, with occasional formation of small 126K bodies. These small 126K bodies moved in the cytoplasmic stream and along the endoplasmic reticulum network. In contrast, co-expression of MP markedly promoted the formation of larger MP-associated 126K bodies, which were generally static but moved occasionally in the cytoplasmic stream. In addition, we found that small, mobile 126K bodies were captured at the terminal part of MP-derived filamentous structures, leading to the formation of 126K-MP complexes and their translocation to another cellular site.

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