Formation of autodiploid strains in Aspergillus niger and their application to citric acid production from starch

Somsak Sarangbin, Satoshi Morikawa, Kotaro Kirimura, Shoji Usami

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Autodiploid strains were induced by colchicine treatment of Aspergillus niger WU-2223L, a citric acid-producing strain. In shaking culture, a representative autodiploid strain, L-d1, yielded higher citric acid than the parental strain, WU-2223L. When glucose was used as a carbon source, L-d1 and WU-2223L produced 67.2 g/l and 62.0 g/l of citric acid, respectively, from 120 g/l of glucose in 9 d-cultivation. Furthermore, the autodiploid strain L-d1 produced 49.6 g/l of citric acid, 1.4 times as much as that produced by WU-2223L from 120 g/l of soluble starch. During the whole period of cultivation with starch, the extracellular glucoamylase activity of L-d1 was on the same level as that of WU-2223L, but the extracellular acid-protease activity of L-d1 was much higher. The addition of pepstatin, an inhibitor of acid protease, to the culture broth at 2 d greatly increased the extracellular glucoamylase activity, and citric acid production by L-d1 reached a level of 59.0 g/l. During several subcultivations on both minimal and complete agar media, the autodiploid strains were genetically stable since they formed diploid conidia in their uniform colonies without producing sectors, and maintained citric acid productivity. However, when cultivated on minimal and complete agar media containing benomyl as a haploidizing reagent, the autodiploid strains readily formed sectors of haploid segregants. The properties of the haploid strains obtained by the benomyl treatment of the autodiploid strains were similar in morphology and citric acid productivity to those of the parental strain, WU-2223L. These results indicated that the enhanced production of citric acid from soluble starch by the autodiploid strains was due to autodiploid formation but not to gene mutation caused by the colchicine treatment.

    Original languageEnglish
    Pages (from-to)474-478
    Number of pages5
    JournalJournal of Fermentation and Bioengineering
    Volume77
    Issue number5
    DOIs
    Publication statusPublished - 1994

    Fingerprint

    Aspergillus niger
    Aspergillus
    Citric acid
    Starch
    Citric Acid
    Benomyl
    Glucan 1,4-alpha-Glucosidase
    Haploidy
    Colchicine
    Agar
    Glucose
    Acids
    Fungal Spores
    Peptide Hydrolases
    Productivity
    Protease Inhibitors
    Diploidy
    Carbon
    Mutation
    Genes

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Bioengineering

    Cite this

    Formation of autodiploid strains in Aspergillus niger and their application to citric acid production from starch. / Sarangbin, Somsak; Morikawa, Satoshi; Kirimura, Kotaro; Usami, Shoji.

    In: Journal of Fermentation and Bioengineering, Vol. 77, No. 5, 1994, p. 474-478.

    Research output: Contribution to journalArticle

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