Formosa haliotis sp. Nov., a brown-alga-degrading bacterium isolated from the gut of the abalone haliotis gigantea

Reiji Tanaka, Ilse Cleenwerck, Yukino Mizutani, Shunpei Iehata, Toshiyuki Shibata, Hideo Miyake, Tetsushi Mori, Yutaka Tamaru, Mitsuyoshi Ueda, Peter Bossier, Peter Vandamme

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Four brown-alga-degrading, Gram-stain-negative, aerobic, non-flagellated, gliding and rodshaped bacteria, designated LMG 28520T, LMG 28521, LMG 28522 and LMG 28523, were isolated from the gut of the abalone Haliotis gigantea obtained in Japan. The four isolates had identical random amplified polymorphic DNA patterns and grew optimally at 25 °C, at pH 6.0-9.0 and in the presence of 1.0-4.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences placed the isolates in the genus Formosa with Formosa algae and Formosa arctica as closest neighbours. LMG 28520T and LMG 28522 showed 100 % DNA-DNA relatedness to each other, 16-17 % towards F. algae LMG 28216T and 17-20 % towards F. arctica LMG 28318T; they could be differentiated phenotypically from these established species. The predominant fatty acids of isolates LMG 28520Tand LMG 28522 were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), iso-C15 : 1 G and iso-C15 : 0. Isolate LMG 28520T contained menaquinone-6 (MK-6) as the major respiratory quinone and phosphatidylethanolamine, two unknown aminolipids and an unknown lipid as the major polar lipids. The DNA G+C content was 34.4 mol% for LMG 28520T and 35.5 mol% for LMG 28522. On the basis of their phylogenetic and genetic distinctiveness, and differential phenotypic properties, the four isolates are considered to represent a novel species of the genus Formosa, for which the name Formosa haliotis sp. nov. is proposed. The type strain is LMG 28520T (=NBRC 111189T).

    Original languageEnglish
    Pages (from-to)4388-4393
    Number of pages6
    JournalInternational Journal of Systematic and Evolutionary Microbiology
    Volume65
    Issue number12
    DOIs
    Publication statusPublished - 2015 Dec 1

    Fingerprint

    Haliotis gigantea
    Phaeophyta
    Haliotis
    Phaeophyceae
    brown alga
    abalone
    Taiwan
    algae
    digestive system
    Bacteria
    DNA
    bacterium
    bacteria
    Arctica (Arcticidae)
    lipid
    alga
    phylogenetics
    gliding
    Lipids
    random amplified polymorphic DNA technique

    ASJC Scopus subject areas

    • Microbiology
    • Ecology, Evolution, Behavior and Systematics

    Cite this

    Formosa haliotis sp. Nov., a brown-alga-degrading bacterium isolated from the gut of the abalone haliotis gigantea. / Tanaka, Reiji; Cleenwerck, Ilse; Mizutani, Yukino; Iehata, Shunpei; Shibata, Toshiyuki; Miyake, Hideo; Mori, Tetsushi; Tamaru, Yutaka; Ueda, Mitsuyoshi; Bossier, Peter; Vandamme, Peter.

    In: International Journal of Systematic and Evolutionary Microbiology, Vol. 65, No. 12, 01.12.2015, p. 4388-4393.

    Research output: Contribution to journalArticle

    Tanaka, R, Cleenwerck, I, Mizutani, Y, Iehata, S, Shibata, T, Miyake, H, Mori, T, Tamaru, Y, Ueda, M, Bossier, P & Vandamme, P 2015, 'Formosa haliotis sp. Nov., a brown-alga-degrading bacterium isolated from the gut of the abalone haliotis gigantea', International Journal of Systematic and Evolutionary Microbiology, vol. 65, no. 12, pp. 4388-4393. https://doi.org/10.1099/ijsem.0.000586
    Tanaka, Reiji ; Cleenwerck, Ilse ; Mizutani, Yukino ; Iehata, Shunpei ; Shibata, Toshiyuki ; Miyake, Hideo ; Mori, Tetsushi ; Tamaru, Yutaka ; Ueda, Mitsuyoshi ; Bossier, Peter ; Vandamme, Peter. / Formosa haliotis sp. Nov., a brown-alga-degrading bacterium isolated from the gut of the abalone haliotis gigantea. In: International Journal of Systematic and Evolutionary Microbiology. 2015 ; Vol. 65, No. 12. pp. 4388-4393.
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    abstract = "Four brown-alga-degrading, Gram-stain-negative, aerobic, non-flagellated, gliding and rodshaped bacteria, designated LMG 28520T, LMG 28521, LMG 28522 and LMG 28523, were isolated from the gut of the abalone Haliotis gigantea obtained in Japan. The four isolates had identical random amplified polymorphic DNA patterns and grew optimally at 25 °C, at pH 6.0-9.0 and in the presence of 1.0-4.0 {\%} (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences placed the isolates in the genus Formosa with Formosa algae and Formosa arctica as closest neighbours. LMG 28520T and LMG 28522 showed 100 {\%} DNA-DNA relatedness to each other, 16-17 {\%} towards F. algae LMG 28216T and 17-20 {\%} towards F. arctica LMG 28318T; they could be differentiated phenotypically from these established species. The predominant fatty acids of isolates LMG 28520Tand LMG 28522 were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), iso-C15 : 1 G and iso-C15 : 0. Isolate LMG 28520T contained menaquinone-6 (MK-6) as the major respiratory quinone and phosphatidylethanolamine, two unknown aminolipids and an unknown lipid as the major polar lipids. The DNA G+C content was 34.4 mol{\%} for LMG 28520T and 35.5 mol{\%} for LMG 28522. On the basis of their phylogenetic and genetic distinctiveness, and differential phenotypic properties, the four isolates are considered to represent a novel species of the genus Formosa, for which the name Formosa haliotis sp. nov. is proposed. The type strain is LMG 28520T (=NBRC 111189T).",
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    AU - Tanaka, Reiji

    AU - Cleenwerck, Ilse

    AU - Mizutani, Yukino

    AU - Iehata, Shunpei

    AU - Shibata, Toshiyuki

    AU - Miyake, Hideo

    AU - Mori, Tetsushi

    AU - Tamaru, Yutaka

    AU - Ueda, Mitsuyoshi

    AU - Bossier, Peter

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