Abstract
Four brown-alga-degrading, Gram-stain-negative, aerobic, non-flagellated, gliding and rodshaped bacteria, designated LMG 28520T, LMG 28521, LMG 28522 and LMG 28523, were isolated from the gut of the abalone Haliotis gigantea obtained in Japan. The four isolates had identical random amplified polymorphic DNA patterns and grew optimally at 25 °C, at pH 6.0-9.0 and in the presence of 1.0-4.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences placed the isolates in the genus Formosa with Formosa algae and Formosa arctica as closest neighbours. LMG 28520T and LMG 28522 showed 100 % DNA-DNA relatedness to each other, 16-17 % towards F. algae LMG 28216T and 17-20 % towards F. arctica LMG 28318T; they could be differentiated phenotypically from these established species. The predominant fatty acids of isolates LMG 28520Tand LMG 28522 were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), iso-C15 : 1 G and iso-C15 : 0. Isolate LMG 28520T contained menaquinone-6 (MK-6) as the major respiratory quinone and phosphatidylethanolamine, two unknown aminolipids and an unknown lipid as the major polar lipids. The DNA G+C content was 34.4 mol% for LMG 28520T and 35.5 mol% for LMG 28522. On the basis of their phylogenetic and genetic distinctiveness, and differential phenotypic properties, the four isolates are considered to represent a novel species of the genus Formosa, for which the name Formosa haliotis sp. nov. is proposed. The type strain is LMG 28520T (=NBRC 111189T).
| Original language | English |
|---|---|
| Pages (from-to) | 4388-4393 |
| Number of pages | 6 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 65 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2015 Dec 1 |
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ASJC Scopus subject areas
- Microbiology
- Ecology, Evolution, Behavior and Systematics
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Formosa haliotis sp. Nov., a brown-alga-degrading bacterium isolated from the gut of the abalone haliotis gigantea. / Tanaka, Reiji; Cleenwerck, Ilse; Mizutani, Yukino; Iehata, Shunpei; Shibata, Toshiyuki; Miyake, Hideo; Mori, Tetsushi; Tamaru, Yutaka; Ueda, Mitsuyoshi; Bossier, Peter; Vandamme, Peter.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 65, No. 12, 01.12.2015, p. 4388-4393.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Formosa haliotis sp. Nov., a brown-alga-degrading bacterium isolated from the gut of the abalone haliotis gigantea
AU - Tanaka, Reiji
AU - Cleenwerck, Ilse
AU - Mizutani, Yukino
AU - Iehata, Shunpei
AU - Shibata, Toshiyuki
AU - Miyake, Hideo
AU - Mori, Tetsushi
AU - Tamaru, Yutaka
AU - Ueda, Mitsuyoshi
AU - Bossier, Peter
AU - Vandamme, Peter
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Four brown-alga-degrading, Gram-stain-negative, aerobic, non-flagellated, gliding and rodshaped bacteria, designated LMG 28520T, LMG 28521, LMG 28522 and LMG 28523, were isolated from the gut of the abalone Haliotis gigantea obtained in Japan. The four isolates had identical random amplified polymorphic DNA patterns and grew optimally at 25 °C, at pH 6.0-9.0 and in the presence of 1.0-4.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences placed the isolates in the genus Formosa with Formosa algae and Formosa arctica as closest neighbours. LMG 28520T and LMG 28522 showed 100 % DNA-DNA relatedness to each other, 16-17 % towards F. algae LMG 28216T and 17-20 % towards F. arctica LMG 28318T; they could be differentiated phenotypically from these established species. The predominant fatty acids of isolates LMG 28520Tand LMG 28522 were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), iso-C15 : 1 G and iso-C15 : 0. Isolate LMG 28520T contained menaquinone-6 (MK-6) as the major respiratory quinone and phosphatidylethanolamine, two unknown aminolipids and an unknown lipid as the major polar lipids. The DNA G+C content was 34.4 mol% for LMG 28520T and 35.5 mol% for LMG 28522. On the basis of their phylogenetic and genetic distinctiveness, and differential phenotypic properties, the four isolates are considered to represent a novel species of the genus Formosa, for which the name Formosa haliotis sp. nov. is proposed. The type strain is LMG 28520T (=NBRC 111189T).
AB - Four brown-alga-degrading, Gram-stain-negative, aerobic, non-flagellated, gliding and rodshaped bacteria, designated LMG 28520T, LMG 28521, LMG 28522 and LMG 28523, were isolated from the gut of the abalone Haliotis gigantea obtained in Japan. The four isolates had identical random amplified polymorphic DNA patterns and grew optimally at 25 °C, at pH 6.0-9.0 and in the presence of 1.0-4.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences placed the isolates in the genus Formosa with Formosa algae and Formosa arctica as closest neighbours. LMG 28520T and LMG 28522 showed 100 % DNA-DNA relatedness to each other, 16-17 % towards F. algae LMG 28216T and 17-20 % towards F. arctica LMG 28318T; they could be differentiated phenotypically from these established species. The predominant fatty acids of isolates LMG 28520Tand LMG 28522 were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), iso-C15 : 1 G and iso-C15 : 0. Isolate LMG 28520T contained menaquinone-6 (MK-6) as the major respiratory quinone and phosphatidylethanolamine, two unknown aminolipids and an unknown lipid as the major polar lipids. The DNA G+C content was 34.4 mol% for LMG 28520T and 35.5 mol% for LMG 28522. On the basis of their phylogenetic and genetic distinctiveness, and differential phenotypic properties, the four isolates are considered to represent a novel species of the genus Formosa, for which the name Formosa haliotis sp. nov. is proposed. The type strain is LMG 28520T (=NBRC 111189T).
UR - http://www.scopus.com/inward/record.url?scp=84955438278&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84955438278&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.000586
DO - 10.1099/ijsem.0.000586
M3 - Article
C2 - 26354496
AN - SCOPUS:84955438278
VL - 65
SP - 4388
EP - 4393
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 12
ER -