TY - JOUR

T1 - Fraction estimation of small, dense LDL using autocorrelation function of dynamic light scattering

AU - Trirongjitmoah, Suchin

AU - Sakurai, Toshihiro

AU - Iinaga, Kazuya

AU - Chiba, Hitoshi

AU - Shimizu, Koichi

PY - 2010/3/15

Y1 - 2010/3/15

N2 - Small, dense low-density lipoprotein (sdLDL) in total LDL is strongly related with the cardiovascular risk level, An optical technique using dynamic light scattering (DLS) measurement is useful for point-ofcare testing of sdLDL. However, the sdLDL fraction estimated from the particle size distribution in DLS data is sensitive to noise and artifacts. Therefore, we derived analytical solutions in a closed form to estimate the fraction of scatterers using the autocorrelation function of scattered light from a polydisperse solution. The effect of the undesired large particles can be eliminated by the pre-processing of the autocorrelation function. The proposed technique was verified using latex standard particles and LDL solutions. Results suggest the feasibility of this technique to estimate the sdLDL fraction using optical scattering measurements

AB - Small, dense low-density lipoprotein (sdLDL) in total LDL is strongly related with the cardiovascular risk level, An optical technique using dynamic light scattering (DLS) measurement is useful for point-ofcare testing of sdLDL. However, the sdLDL fraction estimated from the particle size distribution in DLS data is sensitive to noise and artifacts. Therefore, we derived analytical solutions in a closed form to estimate the fraction of scatterers using the autocorrelation function of scattered light from a polydisperse solution. The effect of the undesired large particles can be eliminated by the pre-processing of the autocorrelation function. The proposed technique was verified using latex standard particles and LDL solutions. Results suggest the feasibility of this technique to estimate the sdLDL fraction using optical scattering measurements

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U2 - 10.1364/OE.18.006315

DO - 10.1364/OE.18.006315

M3 - Article

C2 - 20389654

AN - SCOPUS:77949594899

SN - 1094-4087

VL - 18

SP - 6315

EP - 6326

JO - Optics Express

JF - Optics Express

IS - 6

ER -