Fragmentation analysis by superconducting ion detectors in matrix-assisted laser desorption/ionization (MALDI)

M. Ohkubo, Y. Shigeri, T. Kinumi, N. Saito, M. Ukibe, Y. E. Chen, A. Kushino, A. Kurokawa, H. Sato, Shingo Ichimura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Fragmentation of precursor ions of peptides or proteins is very promising for sequencing with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (TOF-MS). Neutral fragments as well as ions produced by post-source decay (PSD) were measured by a superconducting tunnel junction detector in a linear TOF-MS. It has been demonstrated that the PSD fragments are observable as event bands with different kinetic energies at the same TOF as that of the precursor ions of polystyrene with a nominal mass of 200 kDa and bovine serum albumin of 66.43 kDa.

Original languageEnglish
Pages (from-to)779-781
Number of pages3
JournalNuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
Volume559
Issue number2
DOIs
Publication statusPublished - 2006 Apr 14
Externally publishedYes

Fingerprint

Ionization
Desorption
fragmentation
desorption
Detectors
ionization
Mass spectrometry
Lasers
detectors
Ions
mass spectroscopy
matrices
fragments
lasers
ions
sequencing
Tunnel junctions
decay
albumins
tunnel junctions

Keywords

  • Mass spectroscopy
  • Post-source decay
  • Superconducting tunnel junction

ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Instrumentation

Cite this

Fragmentation analysis by superconducting ion detectors in matrix-assisted laser desorption/ionization (MALDI). / Ohkubo, M.; Shigeri, Y.; Kinumi, T.; Saito, N.; Ukibe, M.; Chen, Y. E.; Kushino, A.; Kurokawa, A.; Sato, H.; Ichimura, Shingo.

In: Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 559, No. 2, 14.04.2006, p. 779-781.

Research output: Contribution to journalArticle

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AU - Shigeri, Y.

AU - Kinumi, T.

AU - Saito, N.

AU - Ukibe, M.

AU - Chen, Y. E.

AU - Kushino, A.

AU - Kurokawa, A.

AU - Sato, H.

AU - Ichimura, Shingo

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