Fragmentation analysis by superconducting ion detectors in matrix-assisted laser desorption/ionization (MALDI)

M. Ohkubo, Y. Shigeri, T. Kinumi, N. Saito, M. Ukibe, Y. E. Chen, A. Kushino, A. Kurokawa, H. Sato, S. Ichimura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Fragmentation of precursor ions of peptides or proteins is very promising for sequencing with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (TOF-MS). Neutral fragments as well as ions produced by post-source decay (PSD) were measured by a superconducting tunnel junction detector in a linear TOF-MS. It has been demonstrated that the PSD fragments are observable as event bands with different kinetic energies at the same TOF as that of the precursor ions of polystyrene with a nominal mass of 200 kDa and bovine serum albumin of 66.43 kDa.

Original languageEnglish
Pages (from-to)779-781
Number of pages3
JournalNuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
Volume559
Issue number2
DOIs
Publication statusPublished - 2006 Apr 14

    Fingerprint

Keywords

  • Mass spectroscopy
  • Post-source decay
  • Superconducting tunnel junction

ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Instrumentation

Cite this