F1-ATPase changes its conformations upon phosphate release

Tomoko Masaike, Eiro Muneyuki, Hiroyuki Noji, Kazuhiko Kinosita, Masasuke Yoshida

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Motor proteins, myosin, and kinesin have γ-phosphate sensors in the switch II loop that play key roles in conformational changes that support motility. Here we report that a rotary motor, F1-ATPase, also changes its conformations upon phosphate release. The tryptophan mutation was introduced into Arg-333 in the β subunit of F1-ATPase from thermophilic Bacillus PS3 as a probe of conformational changes. This residue interacts with the switch II loop (residues 308-315) of the β subunit in a nucleotide-bound conformation. The addition of ATP to the mutant F1 subcomplex α3β(R333W)3γ caused transient increase and subsequent decay of the Trp fluorescence. The increase was caused by conformational changes on ATP binding. The rate of decay agreed well with that of phosphate release monitored by phosphate-binding protein assays. This is the first evidence that the β subunit changes its conformation upon phosphate release, which may share a common mechanism of exerting motility with other motor proteins.

Original languageEnglish
Pages (from-to)21643-21649
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number24
DOIs
Publication statusPublished - 2002 Jun 14
Externally publishedYes

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Proton-Translocating ATPases
Conformations
Phosphates
Phosphate-Binding Proteins
Adenosine Triphosphate
Switches
Kinesin
Bacilli
Myosins
Tryptophan
Bacillus
Assays
Proteins
Nucleotides
Fluorescence
Mutation
Sensors

ASJC Scopus subject areas

  • Biochemistry

Cite this

Masaike, T., Muneyuki, E., Noji, H., Kinosita, K., & Yoshida, M. (2002). F1-ATPase changes its conformations upon phosphate release. Journal of Biological Chemistry, 277(24), 21643-21649. https://doi.org/10.1074/jbc.M110297200

F1-ATPase changes its conformations upon phosphate release. / Masaike, Tomoko; Muneyuki, Eiro; Noji, Hiroyuki; Kinosita, Kazuhiko; Yoshida, Masasuke.

In: Journal of Biological Chemistry, Vol. 277, No. 24, 14.06.2002, p. 21643-21649.

Research output: Contribution to journalArticle

Masaike, T, Muneyuki, E, Noji, H, Kinosita, K & Yoshida, M 2002, 'F1-ATPase changes its conformations upon phosphate release', Journal of Biological Chemistry, vol. 277, no. 24, pp. 21643-21649. https://doi.org/10.1074/jbc.M110297200
Masaike T, Muneyuki E, Noji H, Kinosita K, Yoshida M. F1-ATPase changes its conformations upon phosphate release. Journal of Biological Chemistry. 2002 Jun 14;277(24):21643-21649. https://doi.org/10.1074/jbc.M110297200
Masaike, Tomoko ; Muneyuki, Eiro ; Noji, Hiroyuki ; Kinosita, Kazuhiko ; Yoshida, Masasuke. / F1-ATPase changes its conformations upon phosphate release. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 24. pp. 21643-21649.
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