FTZ-F1α is expressed in the developing gonad of frogs

Minoru Takase, Takeshi Nakajima, Masahisa Nakamura

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Fushi tarazu transcription factor-1 (FTZ-F1), a member of the nuclear hormone receptor superfamily, is a regulator for fushi tarazu gene expression in Drosophila. Its expression pattern during organogenesis in vertebrates, however, is not known yet. In this study, we cloned a frog FTZ-F1 homologue (rrFTZ-F1α) and analyzed its expression and localization during gonadal development of the frog Rana rugosa. Cloned rrFTZ-F1α cDNA encoded a protein of 501 amino acids including the regions I-III and FTZ-F1 box that are evolutionally conserved in the FTZ-F1 superfamily. rrFTZ-F1α shared high similarity at the amino acid level with mouse LRH-1 (76%), human FTF (92%), chicken OR2.0 (92%), Xenopus laevis FF1rA (94%) and zebrafish FF1A (82%). Northern blot analysis showed that the rrFTZ-F1α mRNA at a size of 7.4 kb was the most prominent in the testis among various tissues of adult frogs examined. The RT-PCR analysis revealed that the expression of rrFTZ-F1α was weak in the gonad of tadpoles before stage XVI, but it became stronger in the testis of froglets at stage XXV and much higher in the testis of frogs 2 months after metamorphosis. In addition, in situ hybridization analysis revealed that the rrFTZ-F1α gene was transcribed in germ cells except for sperm in the testis, and in oocytes at stage A in the ovary of frogs 2 months after metamorphosis. Together, these results suggest that FTZ-F1α probably plays an important role in differentiation of germ cells in the gonad of frogs in both sexes. Copyright (C) 2000 Elsevier Science B.V.

    Original languageEnglish
    Pages (from-to)195-200
    Number of pages6
    JournalBiochimica et Biophysica Acta - Gene Structure and Expression
    Volume1494
    Issue number1-2
    DOIs
    Publication statusPublished - 2000 Nov 15

    Fingerprint

    Fushi Tarazu Transcription Factors
    Gonads
    Anura
    Testis
    Germ Cells
    Cells
    Amino Acids
    Ranidae
    Cytoplasmic and Nuclear Receptors
    Organogenesis
    Gene expression
    Xenopus laevis
    Zebrafish
    Northern Blotting
    Complementary DNA
    Genes
    Drosophila
    Oocytes
    In Situ Hybridization
    Larva

    Keywords

    • Frog
    • FTZ-F1 protein
    • Gonad
    • In situ hybridization

    ASJC Scopus subject areas

    • Biochemistry
    • Genetics
    • Structural Biology
    • Biophysics

    Cite this

    FTZ-F1α is expressed in the developing gonad of frogs. / Takase, Minoru; Nakajima, Takeshi; Nakamura, Masahisa.

    In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1494, No. 1-2, 15.11.2000, p. 195-200.

    Research output: Contribution to journalArticle

    Takase, Minoru ; Nakajima, Takeshi ; Nakamura, Masahisa. / FTZ-F1α is expressed in the developing gonad of frogs. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 2000 ; Vol. 1494, No. 1-2. pp. 195-200.
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    abstract = "Fushi tarazu transcription factor-1 (FTZ-F1), a member of the nuclear hormone receptor superfamily, is a regulator for fushi tarazu gene expression in Drosophila. Its expression pattern during organogenesis in vertebrates, however, is not known yet. In this study, we cloned a frog FTZ-F1 homologue (rrFTZ-F1α) and analyzed its expression and localization during gonadal development of the frog Rana rugosa. Cloned rrFTZ-F1α cDNA encoded a protein of 501 amino acids including the regions I-III and FTZ-F1 box that are evolutionally conserved in the FTZ-F1 superfamily. rrFTZ-F1α shared high similarity at the amino acid level with mouse LRH-1 (76{\%}), human FTF (92{\%}), chicken OR2.0 (92{\%}), Xenopus laevis FF1rA (94{\%}) and zebrafish FF1A (82{\%}). Northern blot analysis showed that the rrFTZ-F1α mRNA at a size of 7.4 kb was the most prominent in the testis among various tissues of adult frogs examined. The RT-PCR analysis revealed that the expression of rrFTZ-F1α was weak in the gonad of tadpoles before stage XVI, but it became stronger in the testis of froglets at stage XXV and much higher in the testis of frogs 2 months after metamorphosis. In addition, in situ hybridization analysis revealed that the rrFTZ-F1α gene was transcribed in germ cells except for sperm in the testis, and in oocytes at stage A in the ovary of frogs 2 months after metamorphosis. Together, these results suggest that FTZ-F1α probably plays an important role in differentiation of germ cells in the gonad of frogs in both sexes. Copyright (C) 2000 Elsevier Science B.V.",
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