Fully automated DNA extraction from blood using magnetic particles modified with a hyperbranched polyamidoamine dendrimer

Brandon Yoza; Atsushi Arakaki; Kohei Maruyama; Haruko Takeyama; Tadashi Matsunaga

doi:10.1263/jbb.95.21

Fully automated DNA extraction from blood using magnetic particles modified with a hyperbranched polyamidoamine dendrimer

Brandon Yoza, Atsushi Arakaki, Kohei Maruyama, Haruko Takeyama, Tadashi Matsunaga^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

79 Citations (Scopus)

Abstract

Bacterial and artificial magnetic particles were modified using a polyamidoamine (PAMAM) dendrimer and outer shell amines determined. Bacterial magnetic particles were the most consistently modified. Transmission electron microscopic (TEM) analysis showed that the artificial magnetic particles were structurally damaged by the modification process including sonication. Furthermore, laser particle analysis of the magnetite also revealed damage. Small quantities of dendrimer-modified bacterial magnetic particles were used to extract DNA from blood. The efficiency of DNA recovery was consistently about 30 ng of DNA using 2-10 μg of dendrimer-modified bacterial magnetite. This technique was fully automated using newly developed liquid handling robots and bacterial magnetic particles.

Original language	English
Pages (from-to)	21-26
Number of pages	6
Journal	Journal of Bioscience and Bioengineering
Volume	95
Issue number	1
DOIs	https://doi.org/10.1263/jbb.95.21
Publication status	Published - 2003
Externally published	Yes

Keywords

Bacterial magnetic particles
DNA extraction
Dendrimer
Hyperbranched
Polyamidoamine

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1263/jbb.95.21

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title = "Fully automated DNA extraction from blood using magnetic particles modified with a hyperbranched polyamidoamine dendrimer",

abstract = "Bacterial and artificial magnetic particles were modified using a polyamidoamine (PAMAM) dendrimer and outer shell amines determined. Bacterial magnetic particles were the most consistently modified. Transmission electron microscopic (TEM) analysis showed that the artificial magnetic particles were structurally damaged by the modification process including sonication. Furthermore, laser particle analysis of the magnetite also revealed damage. Small quantities of dendrimer-modified bacterial magnetic particles were used to extract DNA from blood. The efficiency of DNA recovery was consistently about 30 ng of DNA using 2-10 μg of dendrimer-modified bacterial magnetite. This technique was fully automated using newly developed liquid handling robots and bacterial magnetic particles.",

keywords = "Bacterial magnetic particles, DNA extraction, Dendrimer, Hyperbranched, Polyamidoamine",

author = "Brandon Yoza and Atsushi Arakaki and Kohei Maruyama and Haruko Takeyama and Tadashi Matsunaga",

note = "Funding Information: This work was funded in part by a Grant-in-Aid for Specially Promoted Research 2, no. 13002005 from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Tetsushi Mori for valuable technical assistance provided throughout this work.",

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AU - Arakaki, Atsushi

AU - Maruyama, Kohei

AU - Takeyama, Haruko

AU - Matsunaga, Tadashi

N1 - Funding Information: This work was funded in part by a Grant-in-Aid for Specially Promoted Research 2, no. 13002005 from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Tetsushi Mori for valuable technical assistance provided throughout this work.

PY - 2003

Y1 - 2003

N2 - Bacterial and artificial magnetic particles were modified using a polyamidoamine (PAMAM) dendrimer and outer shell amines determined. Bacterial magnetic particles were the most consistently modified. Transmission electron microscopic (TEM) analysis showed that the artificial magnetic particles were structurally damaged by the modification process including sonication. Furthermore, laser particle analysis of the magnetite also revealed damage. Small quantities of dendrimer-modified bacterial magnetic particles were used to extract DNA from blood. The efficiency of DNA recovery was consistently about 30 ng of DNA using 2-10 μg of dendrimer-modified bacterial magnetite. This technique was fully automated using newly developed liquid handling robots and bacterial magnetic particles.

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KW - Dendrimer

KW - Hyperbranched

KW - Polyamidoamine

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Fully automated DNA extraction from blood using magnetic particles modified with a hyperbranched polyamidoamine dendrimer

Abstract

Keywords

ASJC Scopus subject areas

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