Fully automated DNA extraction from blood using magnetic particles modified with a hyperbranched polyamidoamine dendrimer

Brandon Yoza, Atsushi Arakaki, Kohei Maruyama, Haruko Takeyama, Tadashi Matsunaga

Research output: Contribution to journalArticlepeer-review

75 Citations (Scopus)

Abstract

Bacterial and artificial magnetic particles were modified using a polyamidoamine (PAMAM) dendrimer and outer shell amines determined. Bacterial magnetic particles were the most consistently modified. Transmission electron microscopic (TEM) analysis showed that the artificial magnetic particles were structurally damaged by the modification process including sonication. Furthermore, laser particle analysis of the magnetite also revealed damage. Small quantities of dendrimer-modified bacterial magnetic particles were used to extract DNA from blood. The efficiency of DNA recovery was consistently about 30 ng of DNA using 2-10 μg of dendrimer-modified bacterial magnetite. This technique was fully automated using newly developed liquid handling robots and bacterial magnetic particles.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume95
Issue number1
DOIs
Publication statusPublished - 2003 Jan 1
Externally publishedYes

Keywords

  • Bacterial magnetic particles
  • DNA extraction
  • Dendrimer
  • Hyperbranched
  • Polyamidoamine

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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