Fully automated immunoassay system of endocrine disrupting chemicals using monoclonal antibodies chemically conjugated to bacterial magnetic particles

Tadashi Matsunaga, Fumiko Ueki, Kimimichi Obata, Hideji Tajima, Tsuyoshi Tanaka, Haruko Takeyama, Yasuhiro Goda, Shigeru Fujimoto

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The development of a rapid and high-throughput detection system for endocrine disrupting chemicals (EDCs) has been required in the recent years. A fully automated immunoassay system was described for the detection of EDCs, such as alkylphenol ethoxylates (APEs), bisphenol A (BPA) and linear alkylbenzene sulfonates (LASs), using monoclonal antibodies chemically conjugated to bacterial magnetic particles (BMPs) and alkaline phosphatase (ALP)-conjugated EDCs. EDC concentrations were evaluated by the decrease in luminescence based on the competitive reaction of EDCs and ALP-conjugated EDCs. Full automation of the BMP-based immunoassay was achieved by using an automated eight-way pipet moving at X-, Y- and Z-direction and a B/F separation unit. B/F separation was performed on the tip surface of eight-way pipet with a retractable magnet mounted close to the pipet tip. Immunoreactions were saturated after 10min, and the assay was completed within 15min. The detection ranges for APE, BPA and LAS were 6.6ppb-66ppm, 2.3ppt-2.3ppm, and 35ppt-35ppm, respectively. This BMP-based immunoassay system has advantages due to its high sensitivity and rapid measurement of samples.

Original languageEnglish
Pages (from-to)75-83
Number of pages9
JournalAnalytica Chimica Acta
Volume475
Issue number1-2
DOIs
Publication statusPublished - 2003 Jan 3
Externally publishedYes

Fingerprint

Endocrine Disruptors
immunoassay
Immunoassay
antibody
Monoclonal Antibodies
sulfonate
phosphatase
Alkaline Phosphatase
Magnets
Automation
luminescence
Luminescence
automation
chemical
particle
Assays
Throughput
assay
detection

Keywords

  • Alkylphenol ethoxylates (APEs)
  • Bacterial magnetic particles (BMPs)
  • Bisphenol A (BPA)
  • Endocrine disrupting chemicals (EDCs)
  • Fully automated immunoassay system
  • Linear alkylbenzene sulfonates (LASs)

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Fully automated immunoassay system of endocrine disrupting chemicals using monoclonal antibodies chemically conjugated to bacterial magnetic particles. / Matsunaga, Tadashi; Ueki, Fumiko; Obata, Kimimichi; Tajima, Hideji; Tanaka, Tsuyoshi; Takeyama, Haruko; Goda, Yasuhiro; Fujimoto, Shigeru.

In: Analytica Chimica Acta, Vol. 475, No. 1-2, 03.01.2003, p. 75-83.

Research output: Contribution to journalArticle

Matsunaga, Tadashi ; Ueki, Fumiko ; Obata, Kimimichi ; Tajima, Hideji ; Tanaka, Tsuyoshi ; Takeyama, Haruko ; Goda, Yasuhiro ; Fujimoto, Shigeru. / Fully automated immunoassay system of endocrine disrupting chemicals using monoclonal antibodies chemically conjugated to bacterial magnetic particles. In: Analytica Chimica Acta. 2003 ; Vol. 475, No. 1-2. pp. 75-83.
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