Fully automated immunoassay system of endocrine disrupting chemicals using monoclonal antibodies chemically conjugated to bacterial magnetic particles

Tadashi Matsunaga*, Fumiko Ueki, Kimimichi Obata, Hideji Tajima, Tsuyoshi Tanaka, Haruko Takeyama, Yasuhiro Goda, Shigeru Fujimoto

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)

Abstract

The development of a rapid and high-throughput detection system for endocrine disrupting chemicals (EDCs) has been required in the recent years. A fully automated immunoassay system was described for the detection of EDCs, such as alkylphenol ethoxylates (APEs), bisphenol A (BPA) and linear alkylbenzene sulfonates (LASs), using monoclonal antibodies chemically conjugated to bacterial magnetic particles (BMPs) and alkaline phosphatase (ALP)-conjugated EDCs. EDC concentrations were evaluated by the decrease in luminescence based on the competitive reaction of EDCs and ALP-conjugated EDCs. Full automation of the BMP-based immunoassay was achieved by using an automated eight-way pipet moving at X-, Y- and Z-direction and a B/F separation unit. B/F separation was performed on the tip surface of eight-way pipet with a retractable magnet mounted close to the pipet tip. Immunoreactions were saturated after 10min, and the assay was completed within 15min. The detection ranges for APE, BPA and LAS were 6.6ppb-66ppm, 2.3ppt-2.3ppm, and 35ppt-35ppm, respectively. This BMP-based immunoassay system has advantages due to its high sensitivity and rapid measurement of samples.

Original languageEnglish
Pages (from-to)75-83
Number of pages9
JournalAnalytica Chimica Acta
Volume475
Issue number1-2
DOIs
Publication statusPublished - 2003 Jan 3
Externally publishedYes

Keywords

  • Alkylphenol ethoxylates (APEs)
  • Bacterial magnetic particles (BMPs)
  • Bisphenol A (BPA)
  • Endocrine disrupting chemicals (EDCs)
  • Fully automated immunoassay system
  • Linear alkylbenzene sulfonates (LASs)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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