Fully automated on-chip imaging flow cytometry system with disposable contamination-free plastic re-cultivation chip

Masahito Hayashi, Akihiro Hattori, Hyonchol Kim, Hideyuki Terazono, Tomoyuki Kaneko, Kenji Yasuda

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have developed a novel imaging cytometry system using a poly(methyl methacrylate (PMMA)) based microfluidic chip. The system was contamination-free, because sample suspensions contacted only with a flammable PMMA chip and no other component of the system. The transparency and low-fluorescence of PMMA was suitable for microscopic imaging of cells flowing through microchannels on the chip. Sample particles flowing through microchannels on the chip were discriminated by an image-recognition unit with a high-speed camera in real time at the rate of 200 event/s, e.g., microparticles 2.5 μm and 3.0 μm in diameter were differentiated with an error rate of less than 2%. Desired cells were separated automatically from other cells by electrophoretic or dielectrophoretic force one by one with a separation efficiency of 90%. Cells in suspension with fluorescent dye were separated using the same kind of microfluidic chip. Sample of 5 μL with 1 × 10 6 particle/mL was processed within 40 min. Separated cells could be cultured on the microfluidic chip without contamination. The whole operation of sample handling was automated using 3D micropipetting system. These results showed that the novel imaging flow cytometry system is practically applicable for biological research and clinical diagnostics.

Original languageEnglish
Pages (from-to)3618-3634
Number of pages17
JournalInternational journal of molecular sciences
Volume12
Issue number6
DOIs
Publication statusPublished - 2011 Jun 1
Externally publishedYes

Keywords

  • Cell sorting using microgel electrophoresis
  • Imaging cytometry
  • On-chip cytometry

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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